https://scholars.lib.ntu.edu.tw/handle/123456789/495557
標題: | Anti-GAD65 autoantibody in Taiwanese patients with insulin-dependent diabetes mellitus: Effect of HLA on anti-GAD65 positivity and clinical characteristics | 作者: | LEE-MING CHUANG Lin C.-Y. HUEY-PEIR WU Tsai W.-Y. TONG-YUAN TAI Lin B.J. |
公開日期: | 1997 | 出版社: | Blackwell Publishing Ltd | 卷: | 47 | 期: | 4 | 起(迄)頁: | 455-461 | 來源出版物: | Clinical Endocrinology | 摘要: | OBJECTIVE: Anti-GAD65 antibody has been studied widely in patients with insulin-dependent diabetes mellitus (IDDM) in many different populations. However, the prevalence of GAD65 autoantibody has not been assessed in Taiwanese patients with IDDM. We therefore characterized GAD65 antibody and investigated the effect of HLA-DR phenotypes on GAD65 autoimmunity and other clinical characteristics in Taiwanese subjects with IDDM. SUBJECTS AND MEASUREMENTS: Two hundred and twenty-five patients (male 102, female 123) with IDDM were recruited. The diagnostic criteria for IDDM were age of onset before 30 years, presence of diabetic ketoacidosis, and insulin-dependency within 3 years of onset. We employed a radioligand method to detect GAD65 antibody. HLA-DR typing was performed by the PCR-SSO techniques. Plasma C-peptide and anti-thyroid microsomal antibody were also measured. RESULTS: The prevalence of GAD65 antibody according to duration of disease were 50/91 (54.9%), 37/95 (38.9%), 8/24 (33%), and 3/15 (20%) among the groups of duration ?5, 6-10, 11-15, and >15 years, respectively (p=8.0011). There were no significant differences between GAD(+) and GAD(-) patients in age of onset (11.5±6.5 and 11.6±13.4 years, respectively), gender distribution (male:female 39:59 and 58:69, respectively) and percentage with residual cell function (38.8% and 29.1%, respectively). Multiple regression analysis revealed that duration of IDDM correlated inversely with residual β cell function. Earlier onset of IDDM correlated with a loss of β cell function and a HLA-DR phenotype containing DR3/4, DR3/3 or DR3/9. CONCLUSIONS: Prevalence of GAD65 autoantibody among Taiwanese subjects with IDDM was negatively correlated with duration of disease. Different determinants in the HLA-DR locus contributed to the clinical onset of IDDM but not to GAD autoimmunity. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030766277&doi=10.1046%2fj.1365-2265.1997.2851090.x&partnerID=40&md5=ec397de066f4546fa887e2b467c6cc78 https://scholars.lib.ntu.edu.tw/handle/123456789/495557 |
ISSN: | 0300-0664 | DOI: | 10.1046/j.1365-2265.1997.2851090.x | SDG/關鍵字: | adolescent; adult; antibody detection; article; child; correlation function; female; gene locus; HLA system; human; insulin dependent diabetes mellitus; major clinical study; male; priority journal; Taiwan; blood; ethnology; genetics; immunology; immunophenotyping; metabolism; onset age; pancreas islet; prevalence; regression analysis; time; autoantibody; autoantigen; C peptide; glutamate decarboxylase; HLA DR antigen; HLA DR3 antigen; HLA-DRB1 antigen; thyroid microsomal antibodies; Adolescent; Age of Onset; Autoantibodies; Autoantigens; C-Peptide; Child; Diabetes Mellitus, Type 1; Female; Glutamate Decarboxylase; HLA-DR Antigens; HLA-DR3 Antigen; Humans; Immunophenotyping; Islets of Langerhans; Male; Prevalence; Regression Analysis; Taiwan; Time Factors |
顯示於: | 醫學系 |
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