https://scholars.lib.ntu.edu.tw/handle/123456789/499521
標題: | Fiber-based 1150-nm femtosecond laser source for the minimally invasive harmonic generation microscopy | 作者: | Huang, J.-Y. Guo, L.-Z. Wang, J.-Z. Li, T.-C. Lee, H.-J. Chiu, P.-K. LUNG-HAN PENG Liu, T.-M. |
關鍵字: | harmonic generation microscopy; photonic crystal fiber; soliton self-frequency-shift | 公開日期: | 2017 | 卷: | 22 | 期: | 3 | 來源出版物: | Journal of Biomedical Optics | 摘要: | Harmonic generation microscopy (HGM) has become one unique tool of optical virtual biopsy for the diagnosis of cancer and the in vivo cytometry of leukocytes. Without labeling, HGM can reveal the submicron features of tissues and cells in vivo. For deep imaging depth and minimal invasiveness, people commonly adopt 1100- to 1300-nm femtosecond laser sources. However, those lasers are typically based on bulky oscillators whose performances are sensitive to environmental conditions. We demonstrate a fiber-based 1150-nm femtosecond laser source, with 6.5-nJ pulse energy, 86-fs pulse width, and 11.25-MHz pulse repetition rate. It was obtained by a bismuth borate or magnesium-doped periodically poled lithium niobate (MgO:PPLN) mediated frequency doubling of the 2300-nm solitons, generated from an excitation of 1550-nm femtosecond pulses on a large mode area photonic crystal fiber. Combined with a home-built laser scanned microscope and a tailor-made frame grabber, we achieve a pulse-per-pixel HGM imaging in vivo at a 30-Hz frame rate. This integrated solution has the potential to be developed as a stable HGM system for routine clinical use. ? 2017 Society of Photo-Optical Instrumentation Engineers (SPIE). |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/499521 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85015757498&doi=10.1117%2f1.JBO.22.3.036008&partnerID=40&md5=1919290b9fb6870db72867b11c53e504 |
ISSN: | 10833668 | DOI: | 10.1117/1.JBO.22.3.036008 | SDG/關鍵字: | Crystal whiskers; Electromagnetic pulse; Fibers; Frequency doublers; Harmonic analysis; Harmonic generation; Nonlinear optics; Photonic crystal fibers; Solitons; Textile fibers; Ultrafast lasers; Ultrashort pulses; Environmental conditions; Frequency-doubling; Harmonic generation microscopy; Integrated solutions; Large-mode-area photonic crystal fibers; Minimally invasive; Periodically poled lithium niobate; Soliton self-frequency shift; Pulse repetition rate; devices; diagnostic imaging; laser; light; microscopy; photon; Diagnostic Imaging; Lasers; Light; Microscopy; Photons |
顯示於: | 電機工程學系 |
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