Microfluidic device with dual mechanical cues for cell migration investigation
Journal
Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
Pages
842-845
Date Issued
2013
Author(s)
Tsai, C.-H.
Abstract
Cell migration plays an important role in numerous physiological and pathological conditions, such as angiogenesis, wound healing and cancer metastasis. Understanding the fundamental mechanisms of cell migration is crucial to develop strategies for disease treatment and regenerative medicine. Several biomechanical cues have been well studied about their effects on guiding cell migration. However, the effects of dual or multiple cues on cell migration are barely addressed. In this work, we developed a microfluidic-based device to study the combinatory effects of osmotic and stiffness gradient on cell migration. Computer simulation and experimental validation showed that the device was capable of providing stable osmotic and stiffness gradient to cultured cells at the same time. Preliminary results suggest that our device has a valuable potential in studying cell migration in complex conditions which better recapitulate the complex environmental conditions in vivo. ? 2013 IEEE.
SDGs
Other Subjects
Disease treatment; Environmental conditions; Experimental validations; Fundamental mechanisms; Micro-fluidic devices; Microfluidic-based; Pathological conditions; Regenerative medicine; Computer simulation; Osmosis; Stiffness; Diseases; acrylic acid resin; polyacrylamide; sucrose; animal; article; cell culture; cell motion; chemistry; computer simulation; cytology; equipment; mechanics; methodology; microfluidic analysis; mouse; myoblast; Acrylic Resins; Animals; Cell Movement; Cells, Cultured; Computer Simulation; Mechanical Phenomena; Mice; Microfluidic Analytical Techniques; Myoblasts; Sucrose
Type
conference paper