Ultra-highly sensitive and wash-free bio-detection of H5N1 virus by immunomagnetic reduction assays
Journal
Journal of Virological Methods
Journal Volume
153
Journal Issue
2
Pages
250-252
Date Issued
2008
Author(s)
Yang, S.Y.
Chieh, J.J.
Wang, W.C.
Yu, C.Y.
Lan, C.B.
Chen, J.H.
Horng, H.E.
Hong, C.-Y.
Yang, H.C.
Huang, W.
Abstract
A platform for assaying avian influenza H5N1 viruses that involves measuring the ac immunomagnetic reduction of a magnetic reagent mixed with a detected sample is developed. The magnetic reagent contained magnetic nanoparticles coated with antibodies. To achieve an ultra-high sensitivity assay, a system utilizing a high-transition-temperature superconducting quantum interference device was used to sense the immunomagnetic reduction of the reagents. The results confirmed the ultra-high sensitivity of the immunomagnetic reduction assay on H5N1.
Type
journal article