Curcumin inhibits SK-Hep-1 hepatocellular carcinoma cell invasion in vitro and suppresses matrix metalloproteinase-9 secretion
Journal
Oncology
Journal Volume
55
Journal Issue
4
Pages
349-353
Date Issued
1998
Author(s)
Abstract
Curcumin (diferuloylmethane), widely used as a spice and coloring agent in food, possesses potent antioxidant, anti-inflammatory and anticarcinogenic properties. Recently, curcumin was further demonstrated to have an antimetastatic effect in mice. In this study, we attempted to clarify the possible mechanisms of this latter effect of curcumin. A highly invasive SK-Hep-1 cell line of human hepatocellular carcinoma (HCC) was selected for this study. An in vitro assay, without or with Matrigel matrix, was used to quantitate cellular migration and invasion. Gelatin-based zymography was adapted to assay the secretion of matrix metalloproteinase (MMP). We found that curcumin, at 10 μM, inhibited 17.4 and 70.6% of cellular migration and invasion of SK-Hep-1, respectively. Compared with a less invasive human HCC cell line, Huh-7, SK-Hep-1 showed a much higher MMP-9 secretion. Further, and parallel with its anti-invasion activity, curcumin inhibited MMP-9 secretion in SK-Hep-1 in a dose-dependent fashion. We conclude that curcumin has a significant anti-invasion activity in SK-Hep-1 cells, and that this effect is associated with its inhibitory action on MMP-9 secretion.
SDGs
Other Subjects
curcumin; gelatinase b; matrigel; article; cancer inhibition; cancer invasion; controlled study; dose response; drug effect; drug mechanism; enzyme release; human; human cell; liver cell carcinoma; priority journal; Antineoplastic Agents; Carcinoma, Hepatocellular; Collagenases; Curcumin; Dose-Response Relationship, Drug; Humans; Liver Neoplasms; Matrix Metalloproteinase 9; Neoplasm Invasiveness; Tumor Cells, Cultured
Type
journal article