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  4. Liquid biopsy for detection of EGFR T790M mutation in nonsmall cell lung cancer: An experience of proficiency testing in Taiwan
 
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Liquid biopsy for detection of EGFR T790M mutation in nonsmall cell lung cancer: An experience of proficiency testing in Taiwan

Journal
Journal of the Chinese Medical Association
Journal Volume
82
Journal Issue
6
Pages
473-476
Date Issued
2019
Author(s)
Ho H.-L.
Huang C.-C.
Ku W.-H.
Ho C.-L.
Lin C.-H.
SUNG-LIANG YU  
Chou T.-Y.
DOI
10.1097/JCMA.0000000000000100
URI
https://scholars.lib.ntu.edu.tw/handle/123456789/502813
Abstract
Background: The use of liquid biopsy to detect epidermal growth factor receptor (EGFR) T790M mutation in nonsmall cell lung cancer (NSCLC) is a promising method to screen patients eligible for third-generation EGFR inhibitors. Proficiency testing (PT) programs involving liquid biopsy are currently lacking. In this study, we conducted a PT program to assess the quality assurance of liquid biopsy tests for detecting EGFR T790M mutation in molecular pathology laboratories in Taiwan. Methods: Whole blood samples (2 mL) with various concentrations of the EGFR T790M mutation were prepared and analyzed in six participating laboratories using their clinically validated assays. Results: For circulating cell-free DNA (cfDNA) isolation, three of the six participating laboratories used the cobas cfDNA Sample Preparation Kit, and three used the QIAamp Circulating Nucleic Acid Kit. For testing platforms, two of the six participating laboratories used mass spectrometry, three used the cobas EGFR mutation test, and one used a laboratory-developed test. There was 100% concordance in detection of all the given concentrations of EGFR T790M mutation between the participating laboratories and different testing platforms. The testing platforms used by all participating laboratories could successfully detect EGFR T790M mutation to an expected frequency of 1%. Conclusion: In this first PT program using liquid biopsy in Taiwan, local clinical laboratories were suitably equipped and proficient in the use of cfDNA to test for the EGFR T790M mutation. Establishing a routine PT system to ensure the reliability and accuracy of liquid biopsy in clinical practice in Taiwan would be helpful. Copyright ? 2019, the Chinese Medical Association.
Subjects
EGFR T790M; Liquid biopsy; Nonsmall cell lung cancer; Proficiency testing
SDGs

[SDGs]SDG3

Other Subjects
cell free nucleic acid; circulating tumor DNA; epidermal growth factor receptor; cell free nucleic acid; EGFR protein, human; epidermal growth factor receptor; Article; blood sampling; diagnostic accuracy; DNA isolation; gene mutation; human; liquid biopsy; mass spectrometry; molecular pathology; non small cell lung cancer; quality control; Taiwan; blood; genetics; good laboratory practice; liquid biopsy; lung tumor; matrix-assisted laser desorption-ionization mass spectrometry; mutation; non small cell lung cancer; procedures; Carcinoma, Non-Small-Cell Lung; Cell-Free Nucleic Acids; ErbB Receptors; Humans; Laboratory Proficiency Testing; Liquid Biopsy; Lung Neoplasms; Mutation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Type
journal article

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To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

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