Oncogenic miR-137 contributes to cisplatin resistance via repressing CASP3 in lung adenocarcinoma
Journal
American Journal of Cancer Research
Journal Volume
6
Journal Issue
6
Pages
1317-1330
Date Issued
2016
Author(s)
Abstract
Although targeted therapy can prolong the survival of non-small cell lung cancer (NSCLC) patients with EGFR mutations, chemotherapy still is the choice for patients with wild-type EGFR or failure in targeted therapy. However, most of the patients will eventually develop chemoresistance. Our previous study showed that miR-137 is a risky microRNA and is associated with poor prognosis in NSCLC patients. Here we investigated the role of miR-137 in cisplatin resistance in lung adenocarcinoma patients. Our data indicated that miR-137 overexpression increases the survival of lung cancer cells exposed to cisplatin and decreases cisplatin-induced apoptosis. Through computational prediction and microarray, we identified caspase-3 (CASP3) as a potential target of miR-137. Luciferase reporter and site-directed mutagenesis assays demonstrated that miR-137 downregulates CASP3 through binding to its 3'-UTR. Moreover, the endogenous CASP3 can be modulated by overexpressing or silencing miR-137 in lung adenocarcinoma cell lines regardless of EGFR status. Suppression of CASP3 by miR-137 provides cancer cells with anti-apoptotic ability, leading to cisplatin resistance. Immunohistochemistry results revealed an inverse correlation between miR-137 and CASP3 expressions in lung adenocarcinoma patients. Together, our data provide a new chemoresistance mechanism in lung adenocarcinoma and a possible target to control chemoresistance in lung adenocarcinoma patients.
Subjects
Caspase 3; Chemoresistance; Cisplatin; Lung adenocarcinoma; MiR-137
SDGs
Other Subjects
caspase 3; cisplatin; epidermal growth factor receptor; microRNA; microRNA 137; unclassified drug; 3' untranslated region; aged; apoptosis; Article; cancer cell; cancer resistance; cell survival; clinical article; controlled study; down regulation; drug exposure; female; gene function; gene overexpression; genotype; human; human cell; immunohistochemistry; lung adenocarcinoma; male; microarray analysis; protein targeting; site directed mutagenesis
Type
journal article