Repository logo
  • English
  • 中文
Log In
Have you forgotten your password?
  1. Home
  2. College of Medicine / 醫學院
  3. Clinical Laboratory Sciences and Medical Biotechnology / 醫學檢驗暨生物技術學系所
  4. R331W missense mutation of oncogene YAP1 is a germline risk allele for lung adenocarcinoma with medical actionability
 
  • Details

R331W missense mutation of oncogene YAP1 is a germline risk allele for lung adenocarcinoma with medical actionability

Journal
Journal of Clinical Oncology
Journal Volume
33
Journal Issue
20
Pages
2303-2310
Date Issued
2015
Author(s)
Chen H.-Y.
SUNG-LIANG YU  
Ho B.-C.
KANG-YI SU  
Hsu Y.-C.
Chang C.-S.
Li Y.-C.
Yang S.-Y.
Hsu P.-Y.
Ho H.
Chang Y.-H.
Chen C.-Y.
Yang H.-I.
Hsu C.-P.
Yang T.-Y.
Chen K.-C.
Hsu K.-H.
Tseng J.-S.
Hsia J.-Y.
Chuang C.-Y.
Yuan S.
Lee M.-H.
Liu C.-H.
Wu G.-I.
Hsiung C.A.
Chen Y.-M.
Wang C.-L.
Huang M.-S.
CHONG-JEN YU  
KUAN-YU CHEN  
Tsai Y.-H.
Su W.-C.
HUEI-WEN CHEN  
Chen J.J.W.
Chen C.-J.
Chang G.-C.
PAN-CHYR YANG  
Li K.-C.
DOI
10.1200/JCO.2014.59.3590
URI
https://scholars.lib.ntu.edu.tw/handle/123456789/502850
Abstract
Purpose: Adenocarcinoma is the most dominant type of lung cancer in never-smoker patients. The risk alleles from genome-wide association studies have small odds ratios and unclear biologic roles. Here we have taken an approach featuring suitable medical actionability to identify alleles with low population frequency but high disease-causing potential. Patients and Methods: Whole-genome sequencing was performed for a family with an unusually high density of lung adenocarcinoma with available DNA from the affected mother, four affected daughters, and one nonaffected son. Candidate risk alleles were confirmed by matrix-assisted laser desorption ionization time of flight mass spectroscopy. Validation was conducted in an external cohort of 1,135 participants without cancer and 1,312 patients with lung adenocarcinoma. Family follow-ups were performed by genotyping the relatives of the original proband and the relatives of the identified risk-allele carriers. Low-dose computed tomography scans of the chest were evaluated for lung abnormalities. Results: YAP1 R331W missense mutation from the original family was identified and validated in the external controls and the cohort with lung adenocarcinoma. The YAP1 mutant-allele carrier frequency was 1.1% in patients with lung adenocarcinoma compared with 0.18% in controls (P = .0095), yielding an odds ratio (adjusted for age, sex, and smoking status) of 5.9. Among the relatives, YAP1-mutant carriers have overwhelmingly higher frequencies of developing lung adenocarcinoma or ground-glass opacity lung lesions than those who do not carry the mutation (10:0 v 1:7; P < .001). YAP1 mutation was shown to increase the colony formation ability and invasion potential of lung cancer cells. Conclusion: These results implicated YAP1 R331W as an allele predisposed for lung adenocarcinoma with high familial penetrance. Low-dose computed tomography scans may be recommended to this subpopulation, which is at high risk for lung cancer, for personalized prevention and health management. ? 2015 by American Society of Clinical Oncology.
SDGs

[SDGs]SDG3

Other Subjects
transcription factor Yap1; phosphoprotein; signal transducing adaptor protein; tumor marker; YAP1 (Yes-associated) protein, human; adult; aged; allele; Article; bioinformatics; cancer cell; cancer growth; cancer risk; chromosome 11q; clinical article; colony formation; computer assisted tomography; controlled study; female; follow up; gene mutation; gene sequence; genetic screening; genotype; germline mutation; human; lung adenocarcinoma; male; matrix assisted laser desorption ionization time of flight mass spectrometry; missense mutation; penetrance; population; priority journal; single nucleotide polymorphism; adenocarcinoma; biology; case control study; gene frequency; genetic association; genetic predisposition; genetics; germline mutation; heredity; infant; lung tumor; mass spectrometry; middle aged; missense mutation; multivariate analysis; nucleotide sequence; pathology; pedigree; personalized medicine; phenotype; procedures; radiography; risk; risk factor; statistical model; Taiwan; very elderly; Adaptor Proteins, Signal Transducing; Adenocarcinoma; Adult; Aged; Aged, 80 and over; Case-Control Studies; Computational Biology; DNA Mutational Analysis; Female; Gene Frequency; Genetic Predisposition to Disease; Genome-Wide Association Study; Germ-Line Mutation; Heredity; Humans; Individualized Medicine; Infant; Logistic Models; Lung Neoplasms; Male; Middle Aged; Multivariate Analysis; Mutation, Missense; Odds Ratio; Pedigree; Penetrance; Phenotype; Phosphoproteins; Risk Factors; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Taiwan; Tomography, X-Ray Computed; Tumor Markers, Biological
Type
journal article

臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

總館學科館員 (Main Library)
醫學圖書館學科館員 (Medical Library)
社會科學院辜振甫紀念圖書館學科館員 (Social Sciences Library)

開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

  • 請確認所上傳的全文是原創的內容,若該文件包含部分內容的版權非匯入者所有,或由第三方贊助與合作完成,請確認該版權所有者及第三方同意提供此授權。
    Please represent that the submission is your original work, and that you have the right to grant the rights to upload.
  • 若欲上傳已出版的全文電子檔,可使用Open policy finder網站查詢,以確認出版單位之版權政策。
    Please use Open policy finder to find a summary of permissions that are normally given as part of each publisher's copyright transfer agreement.
  • 網站簡介 (Quickstart Guide)
  • 使用手冊 (Instruction Manual)
  • 線上預約服務 (Booking Service)
  • 方案一:臺灣大學計算機中心帳號登入
    (With C&INC Email Account)
  • 方案二:ORCID帳號登入 (With ORCID)
  • 方案一:定期更新ORCID者,以ID匯入 (Search for identifier (ORCID))
  • 方案二:自行建檔 (Default mode Submission)
  • 方案三:學科館員協助匯入 (Email worklist to subject librarians)

Built with DSpace-CRIS software - Extension maintained and optimized by 4Science