Repository logo
  • English
  • 中文
Log In
Have you forgotten your password?
  1. Home
  2. College of Medicine / 醫學院
  3. Clinical Laboratory Sciences and Medical Biotechnology / 醫學檢驗暨生物技術學系所
  4. Expression and antigenicity of human immunodeficiency virus type-1 transmembrane protein GP41 in insect cells
 
  • Details

Expression and antigenicity of human immunodeficiency virus type-1 transmembrane protein GP41 in insect cells

Journal
Biochemical and Biophysical Research Communications
Journal Volume
191
Journal Issue
1
Pages
207-213
Date Issued
1993
Author(s)
SUNG-LIANG YU  
Chou M.-J.
Tam M.F.
Lee T.-H.
Syu W.-J.
DOI
10.1006/bbrc.1993.1204
URI
https://scholars.lib.ntu.edu.tw/handle/123456789/502943
Abstract
The HIV-1 transmembrane protein, gp41, is processed together with the envelope glycoprotein, gp120, from the same precursor, gp160, during the virus maturation. We used a baculovirus expression system to demonstrate that gp41 could be properly expressed without the preceding gp120 sequence. Two constructs with slight differences in the N-terminal region of gp41 were generated: one with a deletion of the first 7 hydrophobic residues of gp41, which have been suggested to be in a region important for membrane fusion and penetration, whereas the second with a complete sequence of gp41 except that a nonconserved leucine was substituted with a glutamine during DNA manipulation. Results from Western blotting with specific antisera confirm the gp41 identity. The sizes of gp41 were sensitive to tunicamycin treatment, indicating that N-linked glycosylation did occur. Further immunoblotting analyses with 90 different serum samples from HIV-1 -infected individuals gave similar reaction patterns, suggesting that gp120 as well as the N-terminal region of gp4l are not critical for the expression and antigenecity of gp41. These eucaryotic constructs should provide valuable gp41 sources for detailed characterization of gp41 functions. ? 1993 Academic Press.
SDGs

[SDGs]SDG3

Other Subjects
glycoprotein; membrane protein; amino acid sequence; animal cell; article; cell membrane; gene expression; human immunodeficiency virus; immunoblotting; insect; nonhuman; nucleotide sequence; priority journal; Amino Acid Sequence; Animal; Baculoviridae; Base Sequence; Blotting, Western; Cell Line; Epitopes; Gene Expression; Genetic Vectors; HIV Envelope Protein gp120; HIV Envelope Protein gp41; HIV-1; Insects; Molecular Sequence Data; Mutagenesis, Insertional; Oligodeoxyribonucleotides; Plasmids; Restriction Mapping; Support, Non-U.S. Gov't; Transfection; Tunicamycin
Type
journal article

臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

總館學科館員 (Main Library)
醫學圖書館學科館員 (Medical Library)
社會科學院辜振甫紀念圖書館學科館員 (Social Sciences Library)

開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

  • 請確認所上傳的全文是原創的內容,若該文件包含部分內容的版權非匯入者所有,或由第三方贊助與合作完成,請確認該版權所有者及第三方同意提供此授權。
    Please represent that the submission is your original work, and that you have the right to grant the rights to upload.
  • 若欲上傳已出版的全文電子檔,可使用Open policy finder網站查詢,以確認出版單位之版權政策。
    Please use Open policy finder to find a summary of permissions that are normally given as part of each publisher's copyright transfer agreement.
  • 網站簡介 (Quickstart Guide)
  • 使用手冊 (Instruction Manual)
  • 線上預約服務 (Booking Service)
  • 方案一:臺灣大學計算機中心帳號登入
    (With C&INC Email Account)
  • 方案二:ORCID帳號登入 (With ORCID)
  • 方案一:定期更新ORCID者,以ID匯入 (Search for identifier (ORCID))
  • 方案二:自行建檔 (Default mode Submission)
  • 方案三:學科館員協助匯入 (Email worklist to subject librarians)

Built with DSpace-CRIS software - Extension maintained and optimized by 4Science