Molecular epidemiology of acute HCV infection in HIV–positive patients from Hong Kong, Taipei, Tokyo
Journal
Liver International
Journal Volume
39
Journal Issue
6
Pages
1044-1051
Date Issued
2019
Author(s)
Uemura H.
Wong N.-S.
Chan D.P.C.
Wong B.C.K.
Lin P.-H.
Su L.-H.
Oka S.
Lee S.-S.
Abstract
Background: Acute hepatitis C virus (HCV) infections have been increasingly reported among human immunodeficiency virus (HIV)-positive men who have sex with men (MSM) in the Asia-Pacific region. It remains unknown whether international network of HCV transmission has occurred in this region. Methods: HIV-positive patients with acute HCV infection, defined as HCV seroconversion within a year or documented acute hepatitis with seroconversion, diagnosed in Hong Kong, Taipei and Tokyo during 2010-2016 were included in this molecular epidemiology study. The NS5B region of the HCV genome (365?bp) was amplified using nested polymerase chain reaction and sequenced. Results: Of 234 HIV-positive patients with acute HCV infection, all were male with 94% being MSM. At the diagnosis of acute HCV infection, 73.5% had concurrent sexually transmitted diseases and 88.0% were receiving combination antiretroviral therapy. The most prevalent HCV genotype was 3a, 2a and 1b in Hong Kong, Taipei and Tokyo respectively. Nine independent clusters belonging to five genotypes (1b, 2a, 2c, 3a and 6a) were identified, each of which occurred in one city without overlapping except for one 3a sequence from Taipei that was closely related genetically to the Hong Kong cluster. Conclusions: No international network of HCV transmission was identified among HIV–positive patients in the three Asia-Pacific cities. The transmission dynamics of sexually acquired HCV differed by city, but the risk of intercity clustering should not be ignored. ? 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
Other Subjects
antiretrovirus agent; acute hepatitis C; adult; antiretroviral therapy; Article; female; gene sequence; genotype; Hong Kong; human; Human immunodeficiency virus infected patient; Human immunodeficiency virus infection; Japan; major clinical study; male; men who have sex with men; molecular epidemiology; nested polymerase chain reaction; phylogeny; seroconversion; virus genome; acute disease; classification; clinical trial; Hepacivirus; hepatitis C; middle aged; molecular epidemiology; multicenter study; risk factor; sexual and gender minority; Taiwan; virology; Acute Disease; Adult; Genotype; Hepacivirus; Hepatitis C; HIV Seropositivity; Hong Kong; Humans; Male; Middle Aged; Molecular Epidemiology; Risk Factors; Sexual and Gender Minorities; Taiwan; Tokyo
Type
journal article