https://scholars.lib.ntu.edu.tw/handle/123456789/504713
標題: | The role of GRHL2 and epigenetic remodeling in epithelial–mesenchymal plasticity in ovarian cancer cells | 作者: | Chung V.Y. Tan T.Z. Ye J. Huang, R.-L. Lai H.-C. Kappei D. Wollmann H. Guccione E. RUBY YUN-JU HUANG |
公開日期: | 2019 | 出版社: | Nature Research | 卷: | 2 | 期: | 1 | 起(迄)頁: | 272 | 來源出版物: | Communications Biology | 摘要: | Cancer cells exhibit phenotypic plasticity during epithelial–mesenchymal transition (EMT) and mesenchymal–epithelial transition (MET) involving intermediate states. To study genome-wide epigenetic remodeling associated with EMT plasticity, we integrate the analyses of DNA methylation, ChIP-sequencing of five histone marks (H3K4me1, H3K4me3, H3K27Ac, H3K27me3 and H3K9me3) and transcriptome profiling performed on ovarian cancer cells with different epithelial/mesenchymal states and on a knockdown model of EMT suppressor Grainyhead-like 2 (GRHL2). We have identified differentially methylated CpG sites associated with EMT, found at promoters of epithelial genes and GRHL2 binding sites. GRHL2 knockdown results in CpG methylation gain and nucleosomal remodeling (reduction in permissive marks H3K4me3 and H3K27ac; elevated repressive mark H3K27me3), resembling the changes observed across progressive EMT states. Epigenetic-modifying agents such as 5-azacitidine, GSK126 and mocetinostat further reveal cell state-dependent plasticity upon GRHL2 overexpression. Overall, we demonstrate that epithelial genes are subject to epigenetic control during intermediate phases of EMT/MET involving GRHL2. ? 2019, The Author(s). |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85071183292&doi=10.1038%2fs42003-019-0506-3&partnerID=40&md5=b86f9c41f9a84decb06ff536c1138ddc https://scholars.lib.ntu.edu.tw/handle/123456789/504713 |
ISSN: | 2399-3642 | DOI: | 10.1038/s42003-019-0506-3 | SDG/關鍵字: | DNA binding protein; GRHL2 protein, human; histone; transcription factor; CpG island; DNA methylation; epithelial mesenchymal transition; female; gene knockdown; genetic epigenesis; genetics; human; metabolism; ovary tumor; pathology; physiology; tumor cell line; Cell Line, Tumor; CpG Islands; DNA Methylation; DNA-Binding Proteins; Epigenesis, Genetic; Epithelial-Mesenchymal Transition; Female; Gene Knockdown Techniques; Histones; Humans; Ovarian Neoplasms; Transcription Factors |
顯示於: | 醫學系 |
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