Molecular and phylogenetic analyses suggest an additional hepatitis B virus genotype "I"
Journal
PLoS ONE
Journal Volume
5
Journal Issue
2
Pages
e9297
Date Issued
2010
Author(s)
Abstract
A novel hepatitis B virus (HBV) strain (W29) was isolated from serum samples in the northwest of China. Phylogenetic and distance analyses indicate that this strain is grouped with a series of distinct strains discovered in Vietnam and Laos that have been proposed to be a new genotype I. TreeOrderScan and GroupScan methods were used to study the intergenotype recombination of this special group. Recombination plots and tree maps of W29 and these putative genotype I strains exhibit distinct characteristics that are unexpected in typical genotype C strains of HBV. The amino acids of P gene, S gene, X gene, and C gene of all genotypes (including subtypes) were compared, and eight unique sites were found in genotype I. In vitro and in vivo experiments were also conducted to determine phenotypic characteristics between W29 and other representative strains of different genotypes obtained from China. Secretion of HBsAg in Huh7 cells is uniformly abundant among genotypes A, B, C, and I (W29), but not genotype D. HBeAg secretion is low in genotype I (W29), whose level is close to genotype A and much lower than genotypes B, C, and D. Results from the acute hydrodynamic injection mouse model also exhibit a similar pattern. From an overview of the results, the viral markers of W29 (I1) in Huh7 cells and mice had a more similar level to genotype A than genotype C, although the latter was closer to W29 in distance analysis. All evidence suggests that W29, together with other related strains found in Vietnam and Laos, should be classified into a new genotype. ? 2010 Yu et al.
SDGs
Other Subjects
amino acid; hepatitis B surface antigen; hepatitis B core antigen; hepatitis B surface antigen; hepatitis B(e) antigen; virus DNA; adult; animal experiment; animal model; article; blood sampling; China; controlled study; female; genetic recombination; hepatitis B; Hepatitis B virus; Hepatitis B virus strain W29; human; human cell; hydrodynamics; Laos; morphological trait; mouse; nonhuman; nucleotide sequence; phylogeny; Viet Nam; virus isolation; virus strain; virus typing; amino acid sequence; animal; Bagg albino mouse; blood; chemistry; classification; DNA sequence; genetics; genotype; hepatitis B; immunology; metabolism; molecular genetics; sequence homology; tumor cell line; virology; Hepatitis B virus; Mus; Amino Acid Sequence; Animals; Cell Line, Tumor; China; DNA, Viral; Genotype; Hepatitis B; Hepatitis B Core Antigens; Hepatitis B e Antigens; Hepatitis B Surface Antigens; Hepatitis B virus; Humans; Laos; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Phylogeny; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Vietnam
Publisher
Public Library of Science
Type
journal article