https://scholars.lib.ntu.edu.tw/handle/123456789/506705
標題: | A simplified method for blood feeding, oral infection, and saliva collection of the dengue vector mosquitoes | 作者: | Sri-In C. Weng S.-C. SHIN-HONG SHIAO Tu W.-C. |
公開日期: | 2020 | 出版社: | Public Library of Science | 卷: | 15 | 期: | 5 | 起(迄)頁: | e0233618 | 來源出版物: | PLoS ONE | 摘要: | A simple device using folded Parafilm-M as an artificial blood feeder was designed for studying two important dengue vector mosquitoes, Aedes aegypti and Aedes albopictus. The efficiency of the artificial blood feeder was investigated by comparing the numbers of engorged mosquitoes that fed on the artificial blood feeder versus mice as a live blood source. Significantly more engorged females Aedes aegypti fed on the artificial blood feeder than on mice. In addition, the artificial feeder could serve as a useful apparatus for oral infection via artificial blood meals, and for saliva collection in mosquitoes. Our method enabled us to collect saliva from multiple mosquitoes at once, providing sufficient infected saliva for determination of the virus titer by plaque assay analysis. Our artificial feeder has the advantage that it is simple, inexpensive, and efficient. ? 2020 Public Library of Science. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85085904531&doi=10.1371%2fjournal.pone.0233618&partnerID=40&md5=7e3489974693f002e718bc7274472818 https://scholars.lib.ntu.edu.tw/handle/123456789/506705 |
ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0233618 | SDG/關鍵字: | saliva protein; adult; Aedes aegypti; Aedes albopictus; animal cell; animal experiment; Article; cell culture; controlled study; dengue; Dengue virus 2; egg laying; female; mouse; mouth infection; nonhuman; protein analysis; saliva analysis; sanguivore; viral plaque assay; virus detection; virus load; Aedes; animal; animal food; dengue; Dengue virus; human; isolation and purification; mosquito vector; physiology; saliva; virology; Aedes; Animal Feed; Animals; Dengue; Dengue Virus; Female; Humans; Mice; Mosquito Vectors; Saliva |
顯示於: | 醫學系 |
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