https://scholars.lib.ntu.edu.tw/handle/123456789/506709
標題: | The non-canonical Notch signaling is essential for the control of fertility in Aedes aegypti | 作者: | Chang C.-H. Liu Y.-T. Weng S.-C. Chen I.-Y. PO-NIEN TSAO SHIN-HONG SHIAO |
公開日期: | 2018 | 出版社: | Public Library of Science | 卷: | 12 | 期: | 3 | 起(迄)頁: | e0006307 | 來源出版物: | PLoS Neglected Tropical Diseases | 摘要: | The Notch signaling pathway is a highly evolutionarily-conserved cell-cell signaling pathway that regulates many events during development. It plays a pivotal role in the regulation of fundamental cellular processes, such as cell proliferation, stem cell maintenance, and differentiation during embryonic and adult development. However, functions of Notch signaling in Aedes aegypti, the major mosquito vector for dengue, are largely unknown. In this study, we identified a unique feature of A. aegypti Notch (AaNotch) in the control of the sterile-like phenotype in female mosquitoes. Silencing AaNotch with a reverse genetic approach significantly reduced the fecundity and fertility of the mosquito. Silencing AaNotch also resulted in the prevention of micropyle formation, which led to impaired fertilization. In addition, JNK phosphorylation (a signaling molecule in the non-canonical Notch signaling pathway) was inhibited in the absence of AaNotch. Furthermore, treatment with a JNK inhibitor in the mosquito resulted in impaired fecundity and fertility. Taken together, our results demonstrate that non-canonical Notch signaling is essential for controlling fertility in the A. aegypti mosquito. ? 2018 Chang et al. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85045109083&doi=10.1371%2fjournal.pntd.0006307&partnerID=40&md5=8c5e6021300ab4785ec6bda4b870a1fb https://scholars.lib.ntu.edu.tw/handle/123456789/506709 |
ISSN: | 1935-2727 | DOI: | 10.1371/journal.pntd.0006307 | SDG/關鍵字: | complementary DNA; double stranded RNA; Notch receptor; stress activated protein kinase; insect protein; Janus kinase inhibitor; mitogen activated protein kinase kinase 4; Notch receptor; Aedes aegypti; Article; controlled study; egg laying; egg production; embryo; enzyme phosphorylation; female; fertility; fertilization; gene expression; insect genetics; male; non canonical Notch signaling; nonhuman; Notch signaling; sperm; ultrastructure; Aedes; animal; antagonists and inhibitors; drug effect; fertility; gene silencing; genetics; growth, development and aging; metabolism; ovum; phosphorylation; physiology; reverse genetics; signal transduction; Aedes; Animals; Female; Fertility; Gene Silencing; Insect Proteins; Janus Kinase Inhibitors; MAP Kinase Kinase 4; Ovum; Phosphorylation; Receptors, Notch; Reverse Genetics; Signal Transduction |
顯示於: | 醫學系 |
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