https://scholars.lib.ntu.edu.tw/handle/123456789/506974
標題: | Multi-functionalized carbon dots as theranostic nanoagent for gene delivery in lung cancer therapy | 作者: | Wu Y.-F. Wu H.-C. CHEN-HSIANG KUAN Lin C.-J. Wang L.-W. Chang C.-W. Wang T.-W. |
公開日期: | 2016 | 出版社: | Nature Publishing Group | 卷: | 6 | 起(迄)頁: | 21170 | 來源出版物: | Scientific Reports | 摘要: | Theranostics, an integrated therapeutic and diagnostic system, can simultaneously monitor the real-time response of therapy. Different imaging modalities can combine with a variety of therapeutic moieties in theranostic nanoagents. In this study, a multi-functionalized, integrated theranostic nanoagent based on folate-conjugated reducible polyethylenimine passivated carbon dots (fc-rPEI-Cdots) is developed and characterized. These nanoagents emit visible blue photoluminescence under 360 nm excitation and can encapsulate multiple siRNAs (EGFR and cyclin B1) followed by releasing them in intracellular reductive environment. In vitro cell culture study demonstrates that fc-rPEI-Cdots is a highly biocompatible material and a good siRNA gene delivery carrier for targeted lung cancer treatment. Moreover, fc-rPEI-Cdots/pooled siRNAs can be selectively accumulated in lung cancer cells through receptor mediated endocytosis, resulting in better gene silencing and anti-cancer effect. Combining bioimaging of carbon dots, stimulus responsive property, gene silencing strategy, and active targeting motif, this multi-functionalized, integrated theranostic nanoagent may provide a useful tool and platform to benefit clinicians adjusting therapeutic strategy and administered drug dosage in real time response by monitoring the effect and tracking the development of carcinomatous tissues in diagnostic and therapeutic aspects. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84958279602&doi=10.1038%2fsrep21170&partnerID=40&md5=9cdfa50f409da516e07caa3b8a31a2d6 https://scholars.lib.ntu.edu.tw/handle/123456789/506974 |
ISSN: | 2045-2322 | DOI: | 10.1038/srep21170 | SDG/關鍵字: | biomaterial; carbon; folic acid; imine; nanoparticle; poly(ethylene imine); polyethylene derivative; small interfering RNA; animal; chemistry; disease model; gene expression; gene silencing; gene transfer; genetics; human; Lung Neoplasms; male; materials testing; molecular imaging; mouse; oxidation reduction reaction; particle size; reporter gene; theranostic nanomedicine; transgene; tumor cell line; ultrastructure; Animals; Biocompatible Materials; Carbon; Cell Line, Tumor; Disease Models, Animal; Folic Acid; Gene Expression; Gene Silencing; Gene Transfer Techniques; Genes, Reporter; Humans; Imines; Lung Neoplasms; Male; Materials Testing; Mice; Molecular Imaging; Nanoparticles; Oxidation-Reduction; Particle Size; Polyethylenes; RNA, Small Interfering; Theranostic Nanomedicine; Transgenes |
顯示於: | 醫學系 |
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