https://scholars.lib.ntu.edu.tw/handle/123456789/507131
Title: | Cis-9, trans-11, trans-13-conjugated linolenic acid induces apoptosis and sustained ERK phosphorylation in 3T3-L1 preadipocytes | Authors: | Chou Y.-C. HUI-MIN SU Lai T.-W. Chyuan J.-H. Chao P.-M. |
Keywords: | 3T3-L1; Adipogenesis; Cis-9; ERK/MAPK; Linolenic acid; Trans-11; Trans-13 onjugated linolenic acid | Issue Date: | 2012 | Journal Volume: | 28 | Journal Issue: | 7/8 | Start page/Pages: | 803-811 | Source: | Nutrition | Abstract: | Objective: The aim of this study was to investigate the adipogenic effect of cis-9, trans-11, trans-13-conjugated linolenic acid (c9, t11, t13-CLN), a fatty acid naturally present in bitter melon. Methods: The 3T3-L1 murine preadipocyte cell line was used to test the effect of saponifiables from whole bitter melon and of commercially prepared pure c9, t11, t13-CLN on adipocyte differentiation. The effect of c9, t11, t13. -CLN on 3T3-L1 cell viability was also tested at proliferation, mitotic clonal expansion, and terminal differentiation stages. Results: Compared to the free fatty acid control mixture, the proadipogenic effect on 3T3-L1 was less potent using saponifiables obtained from bitter melon. C9, t11, t13-CLN, unlike its non-conjugated counterpart linolenic acid (LN) or other common fatty acids such as oleic acid or linoleic acid, exerted no proadipogenic effect on 3T3-L1. In contrast to LN displaying no cytotoxic effect at a concentration ?100 μM, c9, t11, t13-CLN caused a dose-dependent reduction in the viability of pre- and postconfluent preadipocytes associated with apoptosis. Sustained ERK/MAPK activation, accompanied by increased peroxisome proliferator-activated receptor γ phosphorylation, was seen in c9, t11, t13-CLN-treated cells at initiation of differentiation. Conclusion: C9, t11, t13-CLN is less adipogenic for 3T3-L1 cells than LN and this is partly due to its apoptotic effect on proliferating preadipocytes and to the sustained ERK phosphorylation seen during mitotic clonal expansion. ? 2012 Elsevier Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84862180021&doi=10.1016%2fj.nut.2011.11.019&partnerID=40&md5=cb4ac98c21ad69bf6791f91590937f25 https://scholars.lib.ntu.edu.tw/handle/123456789/507131 |
ISSN: | 0899-9007 | DOI: | 10.1016/j.nut.2011.11.019 | SDG/Keyword: | cis 9, trans 11, trans 13 conjugated linolenic acid; fatty acid; linolenic acid; mitogen activated protein kinase; peroxisome proliferator activated receptor gamma; unclassified drug; adipocyte; adipogenesis; animal cell; apoptosis; article; cell clone; cell differentiation; cell expansion; cell proliferation; cell strain 3T3; cell viability; concentration response; controlled study; cytotoxicity; enzyme activation; enzyme phosphorylation; Momordica charantia; nonhuman; priority journal; proadipocyte; protein phosphorylation; 3T3-L1 Cells; Adipocytes; Adipogenesis; Animals; Anti-Obesity Agents; Apoptosis; Cell Proliferation; Cell Survival; Extracellular Signal-Regulated MAP Kinases; Fruit; Gene Expression Regulation; Linolenic Acids; Mice; Momordica charantia; Osmolar Concentration; Phosphorylation; PPAR gamma; Protein Processing, Post-Translational; RNA, Messenger; Triglycerides; Cucumis; Murinae |
Appears in Collections: | 生理學科所 |
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