https://scholars.lib.ntu.edu.tw/handle/123456789/507535
標題: | Multipotential mesenchymal stem cells from femoral bone marrow near the site of osteonecrosis | 作者: | Lee, Hsuan-Shu GUAN-TARN HUANG HONGSEN CHIANG Chiou L.-L. MIN-HUEY CHEN Hsieh C.-H. Jiang C.-C. |
公開日期: | 2003 | 出版社: | AlphaMed Press | 卷: | 21 | 期: | 2 | 起(迄)頁: | 190-199 | 來源出版物: | Stem Cells | 摘要: | Stem cell-based therapies for degenerative disorders and injuries are promising in the new era. Multipotential mesenchymal stem cells (MSCs) from bone marrow (BM) are on the leading edge because they are easy to expand in culture while maintaining their multilineage potential. In vitro assessment of the chondrogenic and osteogenic potentials of cultured MSCs has been established, and the BM used in those experiments was exclusively from healthy donors via iliac crest aspiration. It is unknown whether human marrow obtained from femurs also contains these multipotential MSCs We collected marrow from proximal femurs of two patients undergoing total hip replacement surgery for femoral head osteonecrosis and isolated and culture expanded MSCs to about 20 population doublings. These cells were homogeneously positive for β1-integrin. When pelleted into aggregates and cultured in a medium containing transforming growth factor-β3 for 14 days, the cells began to express mRNA for aggrecan and collagen type II and to deposit immunoreactive collagen type II and sulfated proteoglycans in the matrix, hallmarks of chondrogenic differentiation. These MSCs could also be differentiated into osteocytic lineage in vitro, as shown by increased expression of alkaline phosphatase activity and deposition of mineral content onto culture plates. These results indicate that femoral BM obtained during hip surgeries also contained multipotential MSCs. These data imply that direct replacement therapy using MSCs from in situ marrow may be possible in the future and that an MSC bank may be established by using marrow from this approach, bypassing the necessity for iliac marrow aspiration from healthy donors. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037229908&doi=10.1634%2fstemcells.21-2-190&partnerID=40&md5=b461c2a1b77095f10c89fa96b427abd7 https://scholars.lib.ntu.edu.tw/handle/123456789/507535 |
ISBN: | 1066-5099 | DOI: | 10.1634/stemcells.21-2-190 | SDG/關鍵字: | aggrecan; alkaline phosphatase; beta1 integrin; collagen type 2; messenger RNA; transforming growth factor beta3; article; bone development; bone marrow; bone marrow biopsy; case report; cell culture; cell differentiation; cell lineage; chondrogenesis; degenerative disease; enzyme activity; femur; femur head necrosis; human; human cell; human tissue; iliac crest; male; mesenchyme cell; stem cell transplantation; total hip prosthesis |
顯示於: | 醫學系 |
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