https://scholars.lib.ntu.edu.tw/handle/123456789/507646
Title: | Adenovirus expressing interleukin-1 receptor antagonist alleviates allergic airway inflammation in a murine model of asthma | Authors: | Wang C.-C. Fu C.-L. YAO-HSU YANG Lo Y.-C. LI-CHIEH WANG YA-HUI CHUANG Chang D.-M. BOR-LUEN CHIANG |
Issue Date: | 2006 | Journal Volume: | 13 | Journal Issue: | 19 | Start page/Pages: | 1414-1421 | Source: | Gene Therapy | Abstract: | Interleukin-1 (IL-1) is a proinflammatory cytokine and IL-1 receptor antagonist (IL-1ra) is a natural inhibitor that binds to IL-1 receptor type I without inducing signal transduction. It is suggested that IL-1 is required for allergen-specific T helper type 2 cell activation and the development of airway hyper-responsiveness (AHR), but the immunologic effect of exogenous IL-1ra in allergic asthma remains unclear. To examine the effect of IL-1ra on airway inflammation and immunoeffector cells in allergic asthma, recombinant adenovirus expressing human IL-1ra (Ad-hIL-1ra) was delivered intranasally into ovalbumin (OVA)-immunized mice. Single intranasal administration of Ad-hIL-1ra before airway antigen challenge in OVA-immunized mice significantly decreased the severity of AHR and reduced pulmonary infiltration of eosinophils and neutrophils. Suppression of IL-5 and eotaxin with concomitant enhancement of interferon gamma in bronchoalveolar lavage fluid was also noted in OVA-immunized mice by administration of Ad-hIL-1ra. In addition, histological studies showed that Ad-hIL-1ra was able to decrease OVA-induced peribronchial inflammation. Taken together, our results indicated that administration of Ad-hIL-1ra may have therapeutic potential for the immunomodulatory treatment of allergic asthma. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/507646 | ISSN: | 0969-7128 | DOI: | 10.1038/sj.gt.3302798 | SDG/Keyword: | adenovirus vector; eotaxin; gamma interferon; interleukin 1 receptor blocking agent; interleukin 5; ovalbumin; allergic asthma; animal experiment; animal model; animal tissue; antiinflammatory activity; article; controlled study; disease severity; drug megadose; eosinophil; female; gene expression; histopathology; immunomodulation; low drug dose; lung infiltrate; lung lavage; mouse; neutrophil chemotaxis; nonhuman; priority journal; viral gene delivery system; viral gene therapy; virus recombinant; Adenoviridae; Administration, Inhalation; Animals; Asthma; Bronchi; Bronchoalveolar Lavage Fluid; Chemokine CCL11; Chemokines, CC; Female; Gene Therapy; Genetic Engineering; Genetic Vectors; Hypersensitivity; Interferon Type II; Interferon-gamma; Interleukin 1 Receptor Antagonist Protein; Interleukin-5; Lung; Mice; Mice, Inbred BALB C; Models, Animal; Ovalbumin; Th2 Cells; Transduction, Genetic; Adenoviridae; Murinae [SDGs]SDG3 |
Appears in Collections: | 醫學檢驗暨生物技術學系 |
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