https://scholars.lib.ntu.edu.tw/handle/123456789/507693| Title: | Ganoderic acid X, a lanostanoid triterpene, inhibits topoisomerases and induces apoptosis of cancer cells | Authors: | Li C.-H. Chen P.-Y. Chang U.-M. Kan L.-S. WOEI-HORNG FANG Tsai K.-S. Lin S.-B. |
Issue Date: | 2005 | Journal Volume: | 77 | Journal Issue: | 3 | Start page/Pages: | 252-265 | Source: | Life Sciences | Abstract: | Lanostanoid triterpenes isolated from Ganoderma amboinense were found to inhibit the growth of numerous cancer cell lines, and some of them inhibited the activities of topoisomerases I and IIα in vitro. Among the bioactive isolates, one of the most potent triterpene was identified to be 3α-hydroxy-15α-acetoxy-lanosta-7,9(11),24-trien-26-oic acid, ganoderic acid X (GAX). Treatment of human hepatoma HuH-7 cells with GAX caused immediate inhibition of DNA synthesis as well as activation of ERK and JNK mitogen-activated protein kinases, and cell apoptosis. Molecular events of apoptosis including degradation of chromosomal DNA, decrease in the level of Bcl-xL, the disruption of mitochondrial membrane, cytosolic release of cytochrome c and activation of caspase-3 were elucidated. The ability of GAX to inhibit topoisomerases and to sensitize the cancer cells toward apoptosis fulfills the feature of a potential anticancer drug. ? 2005 Elsevier Inc. All rights reserved. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/507693 | ISSN: | 0024-3205 | DOI: | 10.1016/j.lfs.2004.09.045 | metadata.dc.subject.other: | 3alpha hydroxy 15alpha acetoxylanosta 7,9(11),24 trien 26 oic acid; caspase 3; cytochrome c; DNA topoisomerase; DNA topoisomerase (ATP hydrolysing); ganoderma amboinense extract; mitogen activated protein kinase; plant extract; protein bcl xl; stress activated protein kinase; topoisomerase IIalpha; triterpene; unclassified drug; apoptosis; article; cancer cell culture; cancer inhibition; chromosome; controlled study; cytosol; DNA degradation; DNA synthesis inhibition; drug identification; drug isolation; drug potency; drug sensitization; enzyme activation; enzyme activity; enzyme inhibition; enzyme release; Ganoderma; ganoderma amboinense; hepatoma cell; human; human cell; medicinal plant; membrane damage; mitochondrial membrane; molecule; Antigens, Neoplasm; Antineoplastic Agents; Apoptosis; Caspase 3; Caspases; Cell Line, Tumor; Cell Proliferation; DNA Topoisomerases, Type I; DNA Topoisomerases, Type II, Eukaryotic; DNA-Binding Proteins; Enzyme Activation; Extracellular Signal-Regulated MAP Kinases; Ganoderma; Humans; JNK Mitogen-Activated Protein Kinases; Lanosterol; Medicine, Chinese Traditional; Molecular Structure; Neoplasms; Triterpenes; Ganoderma [SDGs]SDG3 |
| Appears in Collections: | 醫學檢驗暨生物技術學系 |
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