https://scholars.lib.ntu.edu.tw/handle/123456789/507710
標題: | Identifying the functions and biomarkers of Codonopsis pilosula and Astragalus membranaceus aqueous extracts in hepatic cells | 作者: | Ko, P.-H. Huang, C.-W. Chang, H.-H. ERIC YAO-YU CHUANG MONG-HSUN TSAI LIANG-CHUAN LAI |
公開日期: | 2019 | 出版社: | BioMed Central Ltd. | 卷: | 14 | 期: | 1 | 起(迄)頁: | 10 | 來源出版物: | Chinese Medicine (United Kingdom) | 摘要: | Background: Homeostasis is a crucial concept used to describe the condition of patients and the roles of herbs in traditional Chinese medicine. Qi-deficiency pattern is one of the conditions when loss of homeostasis and is usually characterized by symptoms including lassitude, spontaneous sweating, and a weak pulse, which are not easy to quantitate. Codonopsis pilosula and Astragalus membranaceus were usually prescribed for carriers with hepatitis and patients with metastatic colon cancer, because these patients tended to experience fatigue. However, crude drugs were prescribed based on the exterior symptoms of patients without controlling clinical setting, such as gender, age, and dietary habits. Limited molecular evidence of using gene expression as the guide for description is available. Therefore, the purpose of this study was to identify potential and objective biomarkers of these two qi-related drugs in a simplified cellular system. Methods: Aqueous extracts of crude qi-tonifying herbs, C. pilosula and A. membranaceus, and that of a qi-consuming drug, Citrus reticulata, were prepared. Human liver cancer HepG2 cells were treated with the extracts of qi-tonifying herbs for 24 h. Differentially expressed genes were identified using microarrays and quantitative RT-PCR (qRT-PCR) and validated in two other hepatocellular cell lines, Huh7 and L-02. Results: A total of 67 differentially expressed probes that responded to both herbs were identified. A pathway analysis revealed that these genes were involved in the development, growth, movement, and viability of the liver cells. Conclusions: After qRT-PCR validation and examination of clinical data from public domains, our results showed that two genes, GDF15 and HMOX1, could serve as biomarkers in liver cells for identifying responses after treatment with C. pilosula and A. membranaceus. ? 2019 The Author(s). |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85063281563&doi=10.1186%2fs13020-019-0233-1&partnerID=40&md5=26dd547a10015b1e0aa004af12ecb9ef https://scholars.lib.ntu.edu.tw/handle/123456789/507710 |
ISSN: | 1749-8546 | DOI: | 10.1186/s13020-019-0233-1 | SDG/關鍵字: | Astragalus membranaceus extract; Codonopsis pilosula extract; evodiamine; aqueous solution; Article; cell growth; cell maturation; cell motion; cell viability; controlled study; DNA probe; drug activity; drug response; GDF15 gene; gene; gene expression; Hep-G2 cell line; HMOX1 gene; Huh-7 cell line; human; human cell; L-02 cell line; liver cell; mandarin; marker gene; plant root; qi; treatment response |
顯示於: | 生理學科所 |
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