https://scholars.lib.ntu.edu.tw/handle/123456789/507745
標題: | ADAM9 up-regulates N-cadherin via miR-218 suppression in lung adenocarcinoma cells | 作者: | Sher Y.-P. Wang L.-J. Chuang L.-L. Tsai M.-H. Kuo T.-T. Huang C.-C. Chuang E.Y. LIANG-CHUAN LAI |
公開日期: | 2014 | 出版社: | Public Library of Science | 卷: | 9 | 期: | 4 | 起(迄)頁: | e94065 | 來源出版物: | PLoS ONE | 摘要: | Lung cancer is the leading cause of cancer death worldwide, and brain metastasis is a major cause of morbidity and mortality in lung cancer. CDH2 (N-cadherin, a mesenchymal marker of the epithelial-mesenchymal transition) and ADAM9 (a type I transmembrane protein) are related to lung cancer brain metastasis; however, it is unclear how they interact to mediate this metastasis. Because microRNAs regulate many biological functions and disease processes (e.g., cancer) by down-regulating their target genes, microRNA microarrays were used to identify ADAM9-regulated miRNAs that target CDH2 in aggressive lung cancer cells. Luciferase assays and western blot analysis showed that CDH2 is a target gene of miR-218. MiR-218 was generated from pri-mir-218-1, which is located in SLIT2, in non-invasive lung adenocarcinoma cells, whereas its expression was inhibited in aggressive lung adenocarcinoma. The down-regulation of ADAM9 up-regulated SLIT2 and miR-218, thus down-regulating CDH2 expression. This study revealed that ADAM9 activates CDH2 through the release of miR-218 inhibition on CDH2 in lung adenocarcinoma. ? 2014 Sher et al. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84899465786&doi=10.1371%2fjournal.pone.0094065&partnerID=40&md5=5e8eb1031959722d2660290106a5da15 https://scholars.lib.ntu.edu.tw/handle/123456789/507745 |
ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0094065 | SDG/關鍵字: | ADAM9 protein; membrane protein; microRNA; microRNA 218; nerve cell adhesion molecule; unclassified drug; 3' untranslated region; ADAM protein; ADAM9 protein, human; cadherin; CDH2 protein, human; leukocyte antigen; membrane protein; microRNA; MIRN218 microRNA, human; ADAM9 gene; article; brain metastasis; carcinoma cell; CDH2 gene; controlled study; down regulation; gene; gene control; gene repression; gene targeting; human; human cell; luciferase assay; lung adenocarcinoma; microarray analysis; morbidity; mortality; nucleotide sequence; upregulation; Western blotting; 3' untranslated region; adenocarcinoma; binding site; cell motion; chemistry; cluster analysis; gene expression profiling; gene expression regulation; genetics; lung tumor; metabolism; nucleotide sequence; pathology; RNA interference; transcription initiation; tumor cell line; 3' Untranslated Regions; ADAM Proteins; Adenocarcinoma; Antigens, CD; Base Sequence; Binding Sites; Cadherins; Cell Line, Tumor; Cell Movement; Cluster Analysis; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Humans; Lung Neoplasms; Membrane Proteins; MicroRNAs; RNA Interference; Transcriptional Activation |
顯示於: | 生理學科所 |
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