https://scholars.lib.ntu.edu.tw/handle/123456789/507753
標題: | MicroRNA-449a Enhances Radiosensitivity in CL1-0 Lung Adenocarcinoma Cells | 作者: | Liu, Y.-J. Lin, Y.-F. Chen, Y.-F. Luo, E.-C. Sher, Y.-P. MONG-HSUN TSAI ERIC YAO-YU CHUANG LIANG-CHUAN LAI |
公開日期: | 2013 | 卷: | 8 | 期: | 4 | 起(迄)頁: | e62383 | 來源出版物: | PLoS ONE | 摘要: | Lung cancer is the leading cause of cancer-related mortality worldwide. Radiotherapy is often applied for treating lung cancer, but it often fails because of the relative non-susceptibility of lung cancer cells to radiation. MicroRNAs (miRNAs) have been reported to modulate the radiosensitivity of lung cancer cells and have the potential to improve the efficacy of radiotherapy. The purpose of this study was to identify a miRNA that can adjust radiosensitivity in lung adenocarcinoma cells. Two lung adenocarcinoma cell lines (CL1-0 and CL1-5) with different metastatic ability and radiosensitivity were used. In order to understand the regulatory mechanisms of differential radiosensitivity in these isogenic tumor cells, both CL1-0 and CL1-5 were treated with 10 Gy radiation, and were harvested respectively at 0, 1, 4, and 24 h after radiation exposure. The changes in expression of miRNA upon irradiation were examined using Illumina Human microRNA BeadChips. Twenty-six miRNAs were identified as having differential expression post-irradiation in CL1-0 or CL1-5 cells. Among these miRNAs, miR-449a, which was down-regulated in CL1-0 cells at 24 h after irradiation, was chosen for further investigation. Overexpression of miR-449a in CL1-0 cells effectively increased irradiation-induced DNA damage and apoptosis, altered the cell cycle distribution and eventually led to sensitization of CL1-0 to irradiation. ? 2013 Liu et al. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84876249943&doi=10.1371%2fjournal.pone.0062383&partnerID=40&md5=a2689011f80a1142fcd439aeeb9446b2 https://scholars.lib.ntu.edu.tw/handle/123456789/507753 |
ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0062383 | SDG/關鍵字: | microRNA; microRNA 449a; unclassified drug; apoptosis; article; cancer cell culture; cell cycle arrest; cell cycle regulation; controlled study; DNA damage; down regulation; gene identification; gene overexpression; human; human cell; lung adenocarcinoma; metastasis potential; microarray analysis; nucleotide sequence; radiation dose; radiation exposure; radiosensitivity; RNA analysis; Adenocarcinoma; Apoptosis; Cell Cycle; Cell Line, Tumor; Cell Survival; DNA Damage; G2 Phase Cell Cycle Checkpoints; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Humans; Lung Neoplasms; MicroRNAs; Radiation Tolerance |
顯示於: | 生理學科所 |
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