Evaluation of performance of human immunodeficiency virus antigen/antibody combination assays in Taiwan
Journal
Journal of Microbiology, Immunology and Infection
Journal Volume
50
Journal Issue
4
Pages
440-447
Date Issued
2017
Author(s)
Chang C.-K.
Kao C.-F.
Lin P.-H.
Huang H.-L.
Ho S.-Y.
Wong K.-C.
Lin B.-C.
Yeh C.-C.
Lee C.-Y.
Lee C.-N.
Yang J.-Y.
Abstract
Background The fourth-generation human immunodeficiency virus (HIV) combination assay, which can simultaneously detect the presence of anti-HIV antibody and HIV antigen, has been shown to shorten the window period in HIV diagnosis compared with the third-generation HIV antibody immunoassay. This study was aimed to determine the performance of HIV combination assays in Taiwan, where the HIV-1 seroprevalence is 0.007% and HIV-2 infection has never been reported. Methods Performance of three fourth-generation HIV Ag/Ab combination assays (Dia.Pro, Wantai, and Bio-Rad) and one third-generation HIV Ab immunoassay (AxSYM HIV 1/2 gO) was assessed. Results A total of 152 specimens, including 86 confirmed HIV-seropositive and 66 HIV-seronegative samples, were used in the study. The sensitivity of four assays varied from 98.8% to 100%, and specificity varied from 98.5% to 100%. Performance of the 75 equivocal samples, the HIV status of which was confirmed later, in terms of negative prediction varied from 81.8% to 87.5%. The Bio-Rad and Dia.Pro assays exhibited higher sensitivity for the detection of p24 antigen among the three fourth-generation HIV combination assays. Conclusion The three fourth-generation HIV Ag/Ab combination assays exhibited better sensitivity, specificity, and negative prediction than the third-generation HIV Ab immunoassay. ? 2015
SDGs
Other Subjects
antigen p24; Human immunodeficiency virus antibody; Human immunodeficiency virus antigen; Human immunodeficiency virus antibody; Human immunodeficiency virus antigen; antibody detection; antigen detection; Article; controlled study; follow up; HIV test; human; Human immunodeficiency virus 1 group M; Human immunodeficiency virus 1 group O; Human immunodeficiency virus 1 infection; Human immunodeficiency virus 2; Human immunodeficiency virus 2 infection; human immunodeficiency virus antigen antibody combination assay; Human immunodeficiency virus infection; immunoassay; immunoassay analyzer; nonhuman; predictive value; sensitivity and specificity; Taiwan; virus load; Western blotting; blood; comparative study; diagnostic test; evaluation study; Human immunodeficiency virus; Human immunodeficiency virus infection; immunoassay; immunology; isolation and purification; procedures; Diagnostic Tests, Routine; HIV; HIV Antibodies; HIV Antigens; HIV Infections; Humans; Immunoassay; Sensitivity and Specificity; Taiwan
Type
journal article