https://scholars.lib.ntu.edu.tw/handle/123456789/508053
標題: | An External Loop Region of Domain III of Dengue Virus Type 2 Envelope Protein Is Involved in Serotype-Specific Binding to Mosquito but Not Mammalian Cells | 作者: | Hung J.-J. Hsieh M.-T. Young M.-J. CHUAN-LIANG KAO King C.-C. Chang W. |
公開日期: | 2004 | 卷: | 78 | 期: | 1 | 起(迄)頁: | 378-388 | 來源出版物: | Journal of Virology | 摘要: | Dengue virus (DV) is a flavivirus and infects mammalian cells through mosquito vectors. This study investigates the roles of domain III of DV type 2 envelope protein (EIII) in DV binding to the host cell. Recombinant EIII interferes with DV infection to BHK21 and C6/36 cells by blocking dengue virion adsorption to these cells. Inhibition of EIII on BHK21 cells was broad with no serotype specificity; however, inhibition of EIII on C6/36 cells was relatively serotype specific. Soluble heparin completely blocks binding of EIII to BHK21 cells, suggesting that domain III binds mainly to cell surface heparan sulfates. This suggestion is supported by the observation that EIII binds very weakly to gro2C and sog9 mutant mammalian cell lines that lack heparan sulfate. In contrast, heparin does not block binding of EIII to mosquito cells. Furthermore, a synthetic peptide that includes amino acids (aa) 380 to 389 of EIII, IGVEPGQLKL, inhibits binding of EIII to C6/36 but not BHK21 cells. This peptide corresponds to a lateral loop region on domain III of E protein, indicating a possible role of this loop in binding to mosquito cells. In summary, these results suggest that EIII plays an important role in binding of DV type 2 to host cells. In addition, EIII interacts with heparan sulfates when binding to BHK21 cells, and a loop region containing aa 380 to 389 of EIII may participate in DV type 2 binding to C6/36 cells. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/508053 | ISSN: | 0022-538X | DOI: | 10.1128/JVI.78.1.378-388.2004 | SDG/關鍵字: | amino acid; heparan sulfate; heparin; synthetic peptide; virus envelope protein; article; cell adhesion; cell line; cell strain BHK; cell surface; Dengue virus; dengue virus 2; disease carrier; insect cell; mammal cell; mosquito; nonhuman; priority journal; protein binding; protein domain; serotype; virion; virus adsorption; virus strain; Amino Acid Sequence; Animals; Binding Sites; Cell Line; Cricetinae; Culicidae; Dengue Virus; Models, Molecular; Receptors, Virus; Serotyping; Structure-Activity Relationship; Viral Envelope Proteins |
顯示於: | 醫學檢驗暨生物技術學系 |
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