https://scholars.lib.ntu.edu.tw/handle/123456789/508176
標題: | Interleukin 23/interleukin 17 axis activated by Mycobacterium avium complex (MAC) is attenuated in patients with MAC-lung disease | 作者: | CHIN-CHUNG SHU JANN-YUAN WANG Wu M.-F. HSIN-CHIH LAI BOR-LUEN CHIANG CHONG-JEN YU |
公開日期: | 2018 | 卷: | 110 | 起(迄)頁: | 7-14 | 來源出版物: | Tuberculosis | 摘要: | Background: Mycobacterium avium complex (MAC)-lung disease (LD) is increasing in patients without human immunodeficiency virus infection. However, data on host vulnerability to MAC-related immune responses, and in particular the interleukin (IL)-23/IL-17 axis, are lacking. Methods: We enrolled 50 patients with MAC-LD, 25 age-matched patients with tuberculosis (TB) and 25 controls. We measured levels of plasma cytokines, and studied IL-12/IL-17 responses in macrophage and lymphocyte activation to MAC. Results: The plasma level of IL-17 in the MAC group was higher than in the TB and control groups. In in-vitro macrophage stimulation, the expression of IL-23 in macrophages was similar in the patients with MAC-LD and controls, although the expression of IL-12 p40 was lower in the patients with MAC-LD. In assays of lymphocyte activation, IL-17 was induced by MAC-primed macrophages, but its level was lower in the patients with MAC-LD and TB than in the controls. The expression of programmed death (PD)-1 receptor was higher in CD4 + IL17A + lymphocytes in the patients with MAC-LD, and the production of IL-17 was significantly increased by blockade of PD-1 and PD-ligand 1. Conclusions: MAC induced a similar expression of IL-23 from macrophages in the patients with MAC-LD compared to the controls, but a lower expression of IL-17 from lymphocytes, which may be through an increased expression of PD-1. The macrophage response of IL-12 p40 was stronger than that of IL-12 p70, and higher in the controls during MAC disease, which may suggest another kind of MAC-related immune evasion. ? 2018 |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/508176 | ISSN: | 1472-9792 | DOI: | 10.1016/j.tube.2018.03.001 | SDG/關鍵字: | CD4 antigen; glyceraldehyde 3 phosphate dehydrogenase; interleukin 12p35; interleukin 12p40; interleukin 17; interleukin 23; interleukin 23p19; programmed death 1 ligand 1; programmed death 1 receptor; tumor necrosis factor; interleukin 17; interleukin 23; adult; Article; clinical article; controlled study; cytokine production; cytokine response; female; human; human cell; in vitro study; lung disease; lymphocyte activation; macrophage; male; middle aged; Mycobacterium avium complex; priority journal; protein blood level; protein expression; tuberculosis; aged; atypical mycobacteriosis; blood; case control study; immune evasion; immunology; lung disease; Mycobacterium avium complex; THP-1 cell line; Aged; Case-Control Studies; Female; Humans; Immune Evasion; Interleukin-17; Interleukin-23; Lung Diseases; Lymphocyte Activation; Macrophages; Male; Middle Aged; Mycobacterium avium Complex; Mycobacterium avium-intracellulare Infection; THP-1 Cells |
顯示於: | 醫學檢驗暨生物技術學系 |
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