https://scholars.lib.ntu.edu.tw/handle/123456789/508351
標題: | Quantification of endogenous metabolites by the postcolumn infused-internal standard method combined with matrix normalization factor in liquid chromatography-electrospray ionization tandem mass spectrometry | 作者: | Liao H.-W. GUAN-YUAN CHEN MING-SHIANG WU WEI-CHIH LIAO Tsai I.-L. CHING-HUA KUO |
公開日期: | 2015 | 卷: | 1375 | 起(迄)頁: | 62-68 | 來源出版物: | Journal of Chromatography A | 摘要: | Quantification of endogenous metabolites has enabled the discovery of biomarkers for diagnosis and provided for an understanding of disease etiology. The standard addition and stable isotope labeled-internal standard (SIL-IS) methods are currently the most widely used approaches to quantifying endogenous metabolites, but both have some limitations for clinical measurement. In this study, we developed a new approach for endogenous metabolite quantification by the postcolumn infused-internal standard (PCI-IS) method combined with the matrix normalization factor (MNF) method. MNF was used to correct the difference in MEs between standard solution and biofluids, and PCI-IS additionally tailored the correction of the MEs for individual samples. Androstenedione and testosterone were selected as test articles to verify this new approach to quantifying metabolites in plasma. The repeatability (n= 4 runs) and intermediate precision (n= 3 days) in terms of the peak area of androstenedione and testosterone at all tested concentrations were all less than 11% relative standard deviation (RSD). The accuracy test revealed that the recoveries were between 95.72% and 113.46%. The concentrations of androstenedione and testosterone in fifty plasma samples obtained from healthy volunteers were quantified by the PCI-IS combined with the MNF method, and the quantification results were compared with the results of the SIL-IS method. The Pearson correlation test showed that the correlation coefficient was 0.98 for both androstenedione and testosterone. We demonstrated that the PCI-IS combined with the MNF method is an effective and accurate method for quantifying endogenous metabolites. ? 2014 Elsevier B.V. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/508351 | ISSN: | 0021-9673 | DOI: | 10.1016/j.chroma.2014.11.073 | SDG/關鍵字: | Biomolecules; Correlation methods; Diagnosis; Electrospray ionization; Isotopes; Liquids; Mass spectrometry; Metabolites; Electrospray ionization tandem mass spectrometry; Endogenous metabolites; Internal standards; Matrix effects; Normalization factors; Liquid chromatography; androstenedione; testosterone; androstenedione; testosterone; analytic method; androstenedione blood level; Article; blood analysis; calibration; electrospray mass spectrometry; human; intermethod comparison; limit of detection; limit of quantitation; liquid chromatography; matrix normalization factor; measurement accuracy; measurement precision; measurement repeatability; metabolite; normal human; postcolumn infused internal standard; quantitative analysis; testosterone blood level; blood; electrospray mass spectrometry; high performance liquid chromatography; metabolomics; procedures; standard; standards; tandem mass spectrometry; validation study; Androstenedione; Chromatography, High Pressure Liquid; Humans; Metabolomics; Reference Standards; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry; Testosterone |
顯示於: | 法醫學科所 |
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