Modulating chemotaxis of lung cancer cells by using electric fields in a microfluidic device
Journal
Biomicrofluidics
Journal Volume
8
Journal Issue
2
Pages
24107
Date Issued
2014
Author(s)
Abstract
We employed direct-current electric fields (dcEFs) to modulate the chemotaxis of lung cancer cells in a microfluidic cell culture device that incorporates both stable concentration gradients and dcEFs. We found that the chemotaxis induced by a 0.5 μM/mm concentration gradient of epidermal growth factor can be nearly compensated by a 360mV/mm dcEF. When the effect of chemical stimulation was balanced by the electrical drive, the cells migrated randomly, and the path lengths were largely reduced. We also demonstrated electrically modulated chemotaxis of two types of lung cancer cells with opposite directions of electrotaxis in this device. ? 2014 AIP Publishing LLC.
SDGs
Other Subjects
Biochemistry; Biological organs; Cell culture; Diseases; Electric fields; Microfluidics; Concentration gradients; Direct current electric fields; Effect of chemicals; Electrical drives; Epidermal growth factors; Lung cancer cells; Micro-fluidic devices; Microfluidic cell culture devices; Cells
Publisher
American Institute of Physics Inc.
Type
journal article