Molecular third-harmonic-generation microscopy through resonance enhancement with absorbing dye
Journal
Optics Letters
Journal Volume
33
Journal Issue
4
Pages
387-389
Date Issued
2008
Author(s)
Abstract
This paper reports a facile and effortless method to realize three-dimensional (3D) molecular third-harmonic-generation (THG) microscopy through the technique of resonance enhancement with absorbing dye. Hematoxylin, a popular absorbing stain, is applied as an example to verify the multiphoton resonant enhancement based on the 1230 nm excitation light and can selectively enhance THG yield at cell nuclear sites in the studied specimens, serving as a cell nucleus contrast agent. It is concluded that combining THG microscopy with the mature staining technique can readily achieve 3D molecular imaging without using fluorescence.
SDGs
Type
journal article