https://scholars.lib.ntu.edu.tw/handle/123456789/511065
標題: | Optical biopsy of fixed human skin with backward-collected optical harmonics signals | 作者: | YI-HUA LIAO CHI-KUANG SUN WEN-JENG LEE Shieh D.-B. Huang H.-Y. Zhang K.Y.-J. Liu H.-L. Sun C.-K. |
公開日期: | 2005 | 卷: | 13 | 期: | 20 | 起(迄)頁: | 8231-8242 | 來源出版物: | Optics Express | 摘要: | Harmonics-based optical microscopy has been widely applied in biomedical researches due to its noninvasiveness to the studied biomaterials. Due to momentum conservation consideration, most previous studies collect harmonics generation signals in a forward geometry, especially for third harmonic generation signals. However, the adopted forward transmission type geometry is not feasible for future clinical diagnosis. In this paper, first virtual biopsy based on backward propagating optical higher harmonics, combining second harmonic and third harmonic, is demonstrated in the fixed human skin specimens. In our study, third harmonic generation can provide morphologic information including the distribution of basal cells while second harmonic generation can provide distribution of collagen fibers in dermis. Therefore, this technique is ideal for future noninvasive in vivo skin disease examination without dye. ? 2005 Optical Society of America. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/511065 | ISSN: | 1094-4087 | DOI: | 10.1364/OPEX.13.008231 | SDG/關鍵字: | Biomedical engineering; Biopsy; Diagnosis; Harmonic generation; Morphology; Optical properties; Collagen fibers; Human skin; Virtual biopsy; Skin |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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