https://scholars.lib.ntu.edu.tw/handle/123456789/511105
標題: | Mice lacking connective tissue growth factor in the forebrain exhibit delayed seizure response, reduced c-fos expression and different microglial phenotype following acute ptz injection | 作者: | Siow, Pei Fen Tsao, Chih Yu Chang, Ho Ching Chen, Chwen Yu Yu, I. Shing Lee, Kuang Yung LI-JEN LEE |
關鍵字: | Endopiriform nucleus | Gene knockout mice | Hippocampus | Neuronal activity | PTZ-induced seizure | 公開日期: | 2-七月-2020 | 卷: | 21 | 期: | 14 | 來源出版物: | International Journal of Molecular Sciences | 摘要: | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Connective tissue growth factor (CTGF) plays important roles in the development and regeneration of the connective tissue, yet its function in the nervous system is still not clear. CTGF is expressed in some distinct regions of the brain, including the dorsal endopiriform nucleus (DEPN) which has been recognized as an epileptogenic zone. We generated a forebrain-specific Ctgf knockout (FbCtgf KO) mouse line in which the expression of Ctgf in the DEPN is eliminated. In this study, we adopted a pentylenetetrazole (PTZ)-induced seizure model and found similar severity and latencies to death between FbCtgf KO and WT mice. Interestingly, there was a delay in the seizure reactions in the mutant mice. We further observed reduced c-fos expression subsequent to PTZ treatment in the KO mice, especially in the hippocampus. While the densities of astrocytes and microglia in the hippocampus were kept constant after acute PTZ treatment, microglial morphology was different between genotypes. Our present study demonstrated that in the FbCtgf KO mice, PTZ failed to increase neuronal activity and microglial response in the hippocampus. Our results suggested that inhibition of Ctgf function may have a therapeutic potential in preventing the pathophysiology of epilepsy. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/511105 | ISSN: | 16616596 | DOI: | 10.3390/ijms21144921 |
顯示於: | 解剖學暨細胞生物學科所 |
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