https://scholars.lib.ntu.edu.tw/handle/123456789/512835
標題: | Pulsed-wave Ultrasound Hyperthermia Enhanced Nanodrug Delivery Combined with Chloroquine Exerts Effective Antitumor Response and Postpones Recurrence | 作者: | Chiang, Chi-Feng Hsu, Yu-Hone Liu, Chih-Chun PO-CHIN LIANG SHI-CHUEN MIAW Win-Li Lin |
公開日期: | 2019 | 出版社: | NATURE PUBLISHING GROUP | 卷: | 9 | 期: | 1 | 來源出版物: | Scientific reports | 摘要: | Autophagy is found to serve as a surviving mechanism for cancer cells. Inhibiting autophagy has been considered as an adjuvant anti-cancer strategy. In this study, we investigated the anti-tumor effect of combining pulsed-wave ultrasound hyperthermia (pUH) enhanced PEGylated liposomal doxorubicin (PLD) delivery with an autophagy inhibitor chloroquine (CQ). BALB/c mice bearing subcutaneous 4T1 tumor received intravenous injection of PLD (10 mg/kg) plus 15-minute on-tumor pUH on Day 5 after tumor implantation and were then fed with CQ (50 mg/kg daily) thereafter. Prolonged suppression of tumor growth was attained with PLD + pUH + CQ treatment, whereas in PLD + pUH group tumors quickly recurred after an initial inhibition. Treatment with CQ monotherapy had no benefit compared to the control group. Immunohistochemical staining and Western blotting showed that autophagy of cancer cells was blocked for the mice receiving CQ. It indicates that PLD + pUH + CQ is a promising strategy to treat cancer for a long-term inhibition. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/512835 | ISSN: | 2045-2322 | DOI: | 10.1038/s41598-019-47345-8 | SDG/關鍵字: | chloroquine; doxorubicin; nanoparticle; animal; Bagg albino mouse; drug delivery system; experimental mammary neoplasm; female; immunology; mouse; pathology; thermotherapy; tumor cell line; ultrasound; Animals; Cell Line, Tumor; Chloroquine; Doxorubicin; Drug Delivery Systems; Female; Hyperthermia, Induced; Mammary Neoplasms, Experimental; Mice; Mice, Inbred BALB C; Nanoparticles; Ultrasonic Waves |
顯示於: | 免疫學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。