The inhibitory effect of superparamagnetic iron oxide nanoparticle (Ferucarbotran) on osteogenic differentiation and its signaling mechanism in human mesenchymal stem cells
Journal
Toxicology and Applied Pharmacology
Journal Volume
245
Journal Issue
2
Pages
272-279
Date Issued
2010
Author(s)
Hsiao J.-K.
Lai I.-Y.
Yang C.-S.
Huang D.-M.
Abstract
Superparamagnetic iron oxide (SPIO) nanoparticles are very useful for monitoring cell trafficking in vivo and distinguish whether cellular regeneration originated from an exogenous cell source, which is a key issue for developing successful stem cell therapies. However, the impact of SPIO labeling on stem cell behavior remains uncertain. Here, we show the inhibitory effect of Ferucarbotran, an ionic SPIO, on osteogenic differentiation and its signaling mechanism in human mesenchymal stem cells. Ferucarbotran caused a dose-dependent inhibition of osteogenic differentiation, abolished the differentiation at high concentration, promoted cell migration, and activated the signaling molecules, β-catenin, a cancer/testis antigen, SSX, and matrix metalloproteinase 2 (MMP2). An iron chelator, desferrioxamine, suppressed all the above Ferucarbotran-induced actions, demonstrating an important role of free iron in the inhibition of osteogenic differentiation that is mediated by the promotion of cell mobilization, involving the activation of a specific signaling pathway. ? 2010 Elsevier Inc.
SDGs
Other Subjects
alkaline phosphatase; beta catenin; cancer testis antigen; deferoxamine; ferucarbotran; gelatinase A; article; bone marrow; cell adhesion; cell differentiation; cell function; cell migration; controlled study; enzyme activity; human; human cell; human cell culture; in vitro study; inhibition kinetics; mesenchymal stem cell; physical chemistry; signal transduction; Western blotting; zymography; beta Catenin; Cell Differentiation; Cell Movement; Dose-Response Relationship, Drug; Ferrosoferric Oxide; Humans; Matrix Metalloproteinase 2; Mesenchymal Stem Cells; Metal Nanoparticles; Osteogenesis; Signal Transduction
Type
journal article