https://scholars.lib.ntu.edu.tw/handle/123456789/516659
標題: | Estimating the Delivery Efficiency of Drug-Loaded Microbubbles in Cancer Cells with Ultrasound Bioluminescence Imaging | 作者: | A-H Liao Y-K Li W-J Lee M-F Wu HAO-LI LIU M-L Kuo |
公開日期: | 2012 | 卷: | 38 | 期: | 11 | 起(迄)頁: | 1938-1948 | 來源出版物: | Ultrasound in Medicine Biology | 摘要: | The application of drug-loaded microbubbles (MBs) in combination with ultrasound (US), which results in an increase in capillary permeability at the site of US-sonication-induced MB destruction, may be an efficient method of localized drug delivery. This study investigated the mechanism underlying the US-mediated release of luciferin-loaded MBs through the blood vessels to targeted cells using an in vivo bioluminescence imaging (BLI) system. The luciferin-loaded MBs comprised an albumin shell with a diameter of 1234 ± 394 nm (mean ± SD) and contained 2.48 × 109 bubbles/mL; within each MB, the concentration of encapsulated luciferin was 1.48 × 10-10 mg/bubble. The loading efficiency of luciferin in MBs was only about 19.8%, while maintaining both the bioluminescence and acoustic properties. In vitro and in vivo BLI experiments were performed to evaluate the US-mediated release of luciferin-loaded MBs. For in vitro results, the increase in light emission of luciferin-loaded albumin-shelled MBs after destruction via US sonication (6.24 ± 0.72 × 107 photons/s) was significantly higher than that in the luciferin-loaded albumin-shelled MBs (3.11 ± 0.33 × 107 photons/s) (p < 0.05). The efficiency of the US-mediated release of luciferin-loaded MBs in 4T1-luc2 tumor-bearing mice was also estimated. The signal intensity of the tumor with US destruction at 3 W/cm2 for 30 s was significantly higher than without US destruction at 3 (p = 0.025), 5 (p = 0.013), 7 (p = 0.012) and 10 (p = 0.032) min after injecting luciferin-loaded albumin-shelled MBs. The delivery efficiency was, thus, improved with US-mediated release, allowing reduction of the total injection dose of luciferin. ? 2012 World Federation for Ultrasound in Medicine & Biology. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/516659 | ISSN: | 3015629 | DOI: | 10.1016/j.ultrasmedbio.2012.07.013 | SDG/關鍵字: | Bioluminescence imaging; Breast cancer cells; Cancer cells; High frequency ultrasounds; In-vitro; In-vivo; Loading efficiency; Microbubbles; Signal intensities; Acoustic properties; Blood vessels; Cells; Drug delivery; Efficiency; Phosphorescence; Tumors; Ultrasonics; Bioluminescence; albumin; luciferase; luciferin; animal experiment; animal model; animal tissue; article; bioluminescence; blood vessel; breast cancer; controlled study; drug delivery system; echography; female; human; human cell; in vitro study; in vivo study; light scattering; microbubble; mouse; nonhuman; priority journal; reporter gene; target cell; ultrasound; Animals; Antineoplastic Agents, Phytogenic; Cell Line, Tumor; Drug Delivery Systems; Electroporation; Female; Luminescent Measurements; Mammary Neoplasms, Experimental; Mice; Microbubbles; Molecular Imaging; Paclitaxel; Ultrasonography |
顯示於: | 電機工程學系 |
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