https://scholars.lib.ntu.edu.tw/handle/123456789/516662
標題: | Low-Pressure Pulsed Focused Ultrasound with Microbubbles Promotes an Anticancer Immunological Response | 作者: | H-L Liu H-Y Hsieh L-A Lu C-W Kang M-F Wu C-Y Lin HAO-LI LIU H-L Liu H-Y Hsieh L-A Lu C-W Kang M-F Wu C-Y Lin 劉浩澧 |
公開日期: | 2012 | 卷: | 10 | 期: | 221 | 起(迄)頁: | 1-12 | 來源出版物: | Journal of Translational Medicine | 摘要: | Background: High-intensity focused-ultrasound (HIFU) has been successfully employed for thermal ablation of tumors in clinical settings. Continuous- or pulsed-mode HIFU may also induce a host antitumor immune response, mainly through expansion of antigen-presenting cells in response to increased cellular debris and through increased macrophage activation/infiltration. Here we demonstrated that another form of focused ultrasound delivery, using low-pressure, pulsed-mode exposure in the presence of microbubbles (MBs), may also trigger an antitumor immunological response and inhibit tumor growth.Methods: A total of 280 tumor-bearing animals were subjected to sonographically-guided FUS. Implanted tumors were exposed to low-pressure FUS (0.6 to 1.4 MPa) with MBs to increase the permeability of tumor microvasculature.Results: Tumor progression was suppressed by both 0.6 and 1.4-MPa MB-enhanced FUS exposures. We observed a transient increase in infiltration of non-T regulatory (non-Treg) tumor infiltrating lymphocytes (TILs) and continual infiltration of CD8+ cytotoxic T-lymphocytes (CTL). The ratio of CD8+/Treg increased significantly and tumor growth was inhibited.Conclusions: Our findings suggest that low-pressure FUS exposure with MBs may constitute a useful tool for triggering an anticancer immune response, for potential cancer immunotherapy. ? 2012 Liu et al.; licensee BioMed Central Ltd. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/516662 | ISSN: | 14795876 | DOI: | 10.1186/1479-5876-10-221 | SDG/關鍵字: | animal cell; animal experiment; animal model; animal tissue; article; cancer inhibition; CD8+ T lymphocyte; controlled study; cytotoxic T lymphocyte; echography; high intensity focused ultrasound; low pressure pulsed focused ultrasound; lymphocytic infiltration; microbubble; microvasculature; mouse; nonhuman; permeability; regulatory T lymphocyte; tumor immunity; tumor microvasculature; Animals; Computer Systems; Disease Progression; Flow Cytometry; Humans; Immunity; Mice; Mice, Inbred BALB C; Microbubbles; Microscopy, Fluorescence; Microvessels; Models, Immunological; Neoplasms; Permeability; Pressure; Temperature; Tumor Microenvironment; Ultrasonics |
顯示於: | 電機工程學系 |
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