Chromosomal localization and partial genomic structure of the human peroxisome proliferator activated receptor-gamma (hPPARγ) gene
Journal
Biochemical and Biophysical Research Communications
Journal Volume
233
Journal Issue
3
Pages
756-759
Date Issued
1997
Author(s)
Beamer B.A.
Negri C.
Gavrilova O.
Rumberger J.M.
Durcan M.J.
Yarnall D.P.
Hawkins A.L.
Griffin C.A.
Burns D.K.
Roth J.
Reitman M.
Shuldiner A.R.
Abstract
We determined the chromosomal localization and partial genomic structure of the coding region of the human PPAR gamma gene (hPPAR gamma), a nuclear receptor important for adipocyte differentiation and function. Sequence analysis and long PCR of human genomic DNA with primers that span putative introns revealed that intron positions and sizes of hPPAR gamma are similar to those previously determined for the mouse PPAR gamma gene[13]. Fluorescent in situ hybridization localized hPPAR gamma to chromosome 3, band 3p25. Radiation hybrid mapping with two independent primer pairs was consistent with hPPAR gamma being within 1.5 Mb of marker D3S1263 on 3p25-p24.2. These sequences of the intron/exon junctions of the 6 coding exons shared by hPPAR gamma 1 and hPPAR gamma 2 will facilitate screening for possible mutations. Furthermore, D3S1263 is a suitable polymorphic marker for linkage analysis to evaluate PPAR gamma's potential contribution to genetic susceptibility to obesity, lipoatrophy, insulin resistance, and diabetes.
SDGs
Publisher
Academic Press Inc.
Type
journal article