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  1. NTU Scholars
  2. 醫學院
  3. 醫學系
Please use this identifier to cite or link to this item: https://scholars.lib.ntu.edu.tw/handle/123456789/517767
Title: Analysis of P. aeruginosa disinfectant sensitivity and microbial adhesions to worn cosmetic contact lenses
Authors: Shen E.P.
Chu H.-S.
Hsieh Y.-T.
WEI-LI CHEN 
SHAN-CHWEN CHANG 
FUNG-RONG HU 
Issue Date: 2020
Journal Volume: 43
Journal Issue: 4
Start page/Pages: 338-344
Source: Contact Lens and Anterior Eye
Abstract: 
Purpose: To compare the sensitivity of two genotypes of P. aeruginosa to various disinfectant solutions and analyze the attached bacteria on worn cosmetic contact lenses (cosCLs). Methods: In this prospective study, healthy volunteers wore etafilcon (brown), nelfilcon (gray), or hilafilcon (black) cosCLs and microbial adhesion analysis was performed. A rub-off test determined pigment dislodgement. Disinfectant sensitivity to Optifree Replenish (Alcon), Optifree Pure Moist (Alcon), Renu Fresh (Bausch & Lomb), and AoSept Plus (Ciba Vision) was tested at various disinfection times and compared between various genotypes and Type III secretion (T3S) system mutants. Results: Of the 1152 cosCLs collected, 364 were culture positive (32%). The highest rate of culture-positive lens was hilafilcon (chi square, P = 0.0001). Hilafilcon also had a significantly greater number of isolates than etafilcon (P < 0.0001). Hilafilcon was the only lens to fail the rub-off test. Cytotoxic strains were significant more resistant to Renu Fresh than were invasive strains, even at 100% of recommended disinfection time (P = 0.0005). Of the tested disinfectants, Renu Fresh was significantly less effective in killing both genotypes of P. aeruginosa compared to AoSept Plus at all time points (25%, 50%, 75%, and 100% recommended disinfection time, P = 0.0001, 0.0001, 0.0005, and 0.0005, respectively). When the T3S system was dysfunctional, mutant strains were all susceptible to disinfectants (P = 0.0001 for both invasive and cytotoxic strains). Conclusion: Pseudomonas species is commonly found on cosCLs of asymptomatic individuals. Wearers of cosCLs that dislodge pigments may be predisposed to microbial contamination. Cytotoxic strains are more resistant to disinfectant solutions, especially to Renu Fresh. P. aeruginosa disinfectant resistance requires a functional T3S system. ? 2019 British Contact Lens Association
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85075388022&doi=10.1016%2fj.clae.2019.10.137&partnerID=40&md5=797f454d3c913a1ec5a923f28d81fe14
https://scholars.lib.ntu.edu.tw/handle/123456789/517767
ISSN: 1367-0484
DOI: 10.1016/j.clae.2019.10.137
SDG/Keyword: contact lens solution; disinfectant agent; contact lens solution; adult; antibiotic sensitivity; Article; bactericidal activity; bacterium adherence; bacterium culture; bacterium identification; controlled study; cytotoxicity; disinfection; female; genotype; human; human experiment; microbial contamination; mutant; nonhuman; normal human; priority journal; prospective study; Pseudomonas aeruginosa; type III secretion system; bacterial count; contact lens; hydrophilic contact lens; Pseudomonas aeruginosa; Colony Count, Microbial; Contact Lens Solutions; Contact Lenses; Contact Lenses, Hydrophilic; Disinfectants; Humans; Prospective Studies; Pseudomonas aeruginosa
[SDGs]SDG3
Appears in Collections:醫學系

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臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

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