https://scholars.lib.ntu.edu.tw/handle/123456789/517820
標題: | Critical appraisal of Ex Vivo expansion of human limbal epithelial stem cells | 作者: | Tseng S.C.G. Chen S.-Y. Shen Y.-C. WEI-LI CHEN FUNG-RONG HU |
關鍵字: | Epithelium; Ex vivo expansion; Limbal stem cell deficiency; Limbus; Ocular surface; Reconstruction; Stem cells | 公開日期: | 2010 | 卷: | 10 | 期: | 9 | 起(迄)頁: | 841-850 | 來源出版物: | Current Molecular Medicine | 摘要: | The stem cells (SCs) of the corneal epithelium located in the limbal basal layer are the ultimate source to maintain corneal epithelial homeostasis. Like other adult tissue-specfic SCs, self renewal and fate decision of limbal SCs are regulated by a specialized in vivo microenvironment, termed "niche". Loss of limbal SCs or dysfunction of the limbal niche renders corneas with a unique clinical disease labeled limbal stem cell deficiency (LSCD). Besides transplantation of autologous or allogeneic limbal SCs or amniotic membrane, a new strategy of treating LSCD is to transplant a bio-engineered graft by expanding limbal SCs ex vivo. Herein, we conduct a critical appraisal of six protocols that have successfully been practiced in treating human patients with LSCD, and identify issues whether niche regulation has been disrupted or maintained during isolation and expansion. Consequently, we propose a future direction that may circumvent the potential pitfalls existing in these conventional protocols by preserving the interaction between limbal SCs and their native niche cells during isolation and expansion. Such an approach may one day help realize considerable promise held by adult SCs in treating a number of diseases. ? 2010 Bentham Science Publishers Ltd. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-78651289159&doi=10.2174%2f156652410793937796&partnerID=40&md5=0cc81f15010274f88d3c8110408d77dc https://scholars.lib.ntu.edu.tw/handle/123456789/517820 |
ISSN: | 1566-5240 | DOI: | 10.2174/156652410793937796 | SDG/關鍵字: | allogeneic stem cell transplantation; amniotic membrane transplantation; article; bioengineering; cell expansion; cell fate; cell isolation; cell renewal; ex vivo study; homeostasis; human; in vivo study; limbal epithelial stem cell; microenvironment; stem cell; Adult Stem Cells; Amnion; Animals; Cell Proliferation; Corneal Diseases; Epithelium, Corneal; Humans; Limbus Corneae; Stem Cell Transplantation; Stem Cells |
顯示於: | 醫學系 |
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