|Title:||Chitosan as an adjuvant-like substrate for dendritic cell culture to enhance antitumor effects||Authors:||Lin Y.-C
|Issue Date:||2014||Publisher:||Elsevier Ltd||Journal Volume:||35||Journal Issue:||31||Start page/Pages:||8867-8875||Source:||Biomaterials||Abstract:||
To induce monocyte differentiation into dendritic cells (DCs) is the essential protocol for the DC-mediated cancer immunotherapy. In this study, monocytes isolated from mouse bone marrow were cultured on chitosan substrate to evaluate the effect of the chitosan culture system on the induction and tumor protection of DCs. Compared to tissue culture polystyrene (TCPS), the chitosan culture system could enhance monocyte aggregation and detachment with increased MTT reduction activity and expression of DC marker CD11c and LPS co-receptor CD14. Moreover, compared to TCPS, chitosan could enhance lipopolysaccharides (LPS)-stimulated DCs to secrete higher amount of IL-12. More importantly, vaccination of tumor lysate-pulsed DCs harvested from chitosan could increase cytotoxic T-lymphocyte (CTL) activity and showed significantly enhanced anti-tumor effect than those from TCPS. Therefore, the current study demonstrated that a protocol to culture DCs on a less-adherent chitosan substrate followed by treatment with tumor lysate has the potential in future DC-based vaccine application. ? 2014 Elsevier Ltd.
|ISSN:||0142-9612||DOI:||10.1016/j.biomaterials.2014.07.014||SDG/Keyword:||Body fluids; Cells; Tissue culture; Tumors; Vaccines; Antitumor effect; Cancer immunotherapy; Dendritic cells; Dendritic cells (DCs); Lipopolysaccharides; Reduction activity; Tissue-culture polystyrenes; Tumor protection; Chitosan; CD14 antigen; chitosan; glycoprotein p 15095; interleukin 12; lipopolysaccharide; biomaterial; cancer vaccine; chitosan; immunological adjuvant; animal cell; animal tissue; antineoplastic activity; article; bone marrow; cell aggregation; controlled study; cytotoxic T lymphocyte; dendritic cell; monocyte; mouse; nonhuman; priority journal; protein expression; animal; C57BL mouse; cell culture; cell differentiation; culture technique; cytology; dendritic cell; drug effects; human; immunology; Neoplasms; pathology; procedures; tumor cell line; Adjuvants, Immunologic; Animals; Biocompatible Materials; Cancer Vaccines; Cell Culture Techniques; Cell Differentiation; Cell Line, Tumor; Cells, Cultured; Chitosan; Dendritic Cells; Humans; Mice; Mice, Inbred C57BL; Monocytes; Neoplasms
|Appears in Collections:||醫學系|
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