https://scholars.lib.ntu.edu.tw/handle/123456789/518251
標題: | Characterization of oral squamous cell carcinoma based on higher-harmonic generation microscopy | 作者: | Tsai M.-R Shieh D.-B PEI-JEN LOU CHIH-FENG LIN Sun C.-K. |
關鍵字: | Harmonic generation microscopy; Histopathological feature; Optical biopsy; Oral squamous cell carcinoma | 公開日期: | 2012 | 卷: | 5 | 期: | 5��6�� | 起(迄)頁: | 415-424 | 來源出版物: | Journal of Biophotonics | 摘要: | In vivo higher-harmonic generation microscopy (HGM) performed on healthy human oral mucosa not only provides images with a <500 nm lateral resolution at a 280 μm penetration depth, but also leaves no photodamages in the tissues. These advantages suggest that HGM could serve as an ideal virtual biopsy tool for in vivo, in situ, and immediate histopathological diagnosis of oral cancer. However, translation of such mechanism for clinical cancer diagnosis requires evidence based algorithm capable to differentiate cancerous tissues from normal. It is thus critical to investigate if the endogenous contrast provided by the HGM would be high enough to differentiate cancerous versus normal tissues in human oral mucosa. In this report, ex vivo HGM study was performed on the cancerous mucosa from 10 patients with oral squamous cell carcinoma. Compared with histology, HGM revealed histopathological features including the cytological abnormalities, loss of differentiation, interruption of basement membrane, and irregular epithelial stratification in all 10 specimens. In addition, distinct patterns of collagen fibers and increased distribution area of actin filaments in tumor cells were noted. These results indicate HGM holds great potential for the optical biopsy screening of oral cancer lesions. ? 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860587337&doi=10.1002%2fjbio.201100144&partnerID=40&md5=769b8ac883d423eab110698d0e149ade https://scholars.lib.ntu.edu.tw/handle/123456789/518251 |
ISSN: | 1864-063X | DOI: | 10.1002/jbio.201100144 | SDG/關鍵字: | Actin filament; Basement membrane; Cancer diagnosis; Cancerous tissues; Collagen fiber; Distribution area; Ex-vivo; Harmonic generation microscopy; Healthy humans; Histopathological diagnosis; Histopathological feature; In-situ; In-vivo; Lateral resolution; Normal tissue; Optical biopsies; Oral cancer; Oral mucosa; Squamous cell carcinoma; Tumor cells; Biopsy; Cytology; Diseases; Harmonic generation; Histology; Proteins; Tissue; Cells; adult; aged; article; cytoplasm; female; human; male; metabolism; methodology; microscopy; middle aged; mouth tumor; pathology; squamous cell carcinoma; Adult; Aged; Cytoplasm; Female; Humans; Male; Microscopy; Middle Aged; Mouth Neoplasms; Neoplasms, Squamous Cell |
顯示於: | 醫學系 |
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