https://scholars.lib.ntu.edu.tw/handle/123456789/518284
標題: | PMMA particle-mediated DNA vaccine for cervical cancer | 作者: | PEI-JEN LOU WEN-FANG CHENG Chung Y.-C Cheng C.-Y Chiu L.-H Tai-Horng Young |
公開日期: | 2009 | 卷: | 88 | 期: | 4 | 起(迄)頁: | 849-857 | 來源出版物: | Journal of Biomedical Materials Research - Part A | 摘要: | DNA vaccination is a novel immunization strategy that possesses many potential advantages over other vaccine strategies. One of the major difficulties hindering the clinical application of DNA vaccination is the relative poor immunogenicity of DNA vaccines. Poly(methyl methacrylate) (PMMA) is a synthetic polymer approved by the Food and Drug Administration for certain human clinical applications such as the bone cement. In vivo, PMMA particles are phagocytosable and have the potential to initiate strong immune responses by stimulating the production of inflammatory cytokines. In this study, we synthesized a series of PMMA particles (PMMA 1-5) with different particle sizes and surface charges to test the feasibility of implementing such polymer particles for DNA vaccination. To our knowledge, this is the first report to show that the gene gun can deliver DNA vaccine by propelling PMMA particles mixed with plasmid DNA for cervical cancer. It was found that PMMA 4 particles (particle size: 460 ± 160 nm, surface charge: +11.5 ± 1.8 mV) stimulated the highest level of TNF-α production by macrophages in vitro and yielded the best result of antitumor protection in vivo. Therefore, our results possess the potential for translation and implementation of polymer particles in gene gun delivering DNA vaccination. ? 2008 Wiley Periodicals, Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-60349128014&doi=10.1002%2fjbm.a.31919&partnerID=40&md5=2d8daf7c4bd3395c5601039c4ae11172 https://scholars.lib.ntu.edu.tw/handle/123456789/518284 |
ISSN: | 1549-3296 | DOI: | 10.1002/jbm.a.31919 | SDG/關鍵字: | Anti tumors; Cervical cancer; Clinical applications; Dna vaccinations; DNA vaccine; Food and drug administrations; Gene gun; Immune response; Immunogenicity; In-vitro; In-vivo; Inflammatory cytokines; PMMA particles; Poly(methyl methacrylate) (PMMA) particles; Polymer particles; Synthetic polymers; Bone cement; Charged particles; DNA; Electron guns; Esters; Genes; Nucleic acids; Organic acids; Particle size; Polymers; Surface charge; Vaccines; bone cement; cytokine; DNA vaccine; plasmid DNA; poly(methyl methacrylate); polymer; tumor necrosis factor alpha; animal cell; animal experiment; animal model; animal tissue; antineoplastic activity; article; cell protection; controlled study; cytokine production; DNA immunization; feasibility study; food and drug administration; gene gun; human; immune response; in vitro study; in vivo study; inflammation; macrophage; mouse; nonhuman; nonviral gene delivery system; particle size; phagocytosis; surface charge; synthesis; uterine cervix cancer; Animals; Cell Line; DNA; Female; Humans; Macrophages; Materials Testing; Mice; Particle Size; Plasmids; Polymethyl Methacrylate; Transfection; Tumor Necrosis Factor-alpha; Uterine Cervical Neoplasms; Vaccines, DNA |
顯示於: | 醫學系 |
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