https://scholars.lib.ntu.edu.tw/handle/123456789/518292
標題: | Application of highly sensitive, modified glass substrate-based immuno-PCR on the early detection of nasopharyngeal carcinoma | 作者: | Wang T.-W Lu H.-Y PEI-JEN LOU Lin, Feng-Huei |
關鍵字: | Cancer; Immuno-PCR; Immunoassay; Silane; Surface modification | 公開日期: | 2008 | 卷: | 29 | 期: | 33 | 起(迄)頁: | 4447-4454 | 來源出版物: | Biomaterials | 摘要: | In this study, we investigated the utilization of highly sensitive immuno-PCR (IPCR) method as a powerful tool to detect NPC in early disease stage. We established a substrate-ELISA platform as a model system for evaluation of the feasibility of our idea after surface modification process on glass beads. Therein the DNA-antibody conjugation was added to sensitize prior enzyme substrate-antibody complex. In the study, the detection efficiency of two different systems regarding sensitivity, affinity, and specificity was evaluated. Moreover, to show the efficacy of our IPCR system, commercialized ELISA kit was also included for comparison with our IPCR glass substrate-based capture system. The surface physical properties of the modified substrates were also tested with atomic force microscopy and X-ray photoelectron spectroscopy, together with the measurement of the water contact angle. In the results, various factors in the production of IPCR detection system were determined to maximize the effect on assay performance, including the modification of the glass surface properties, primary and secondary antibody optimal concentrations, and biotinylated reporter DNA concentration. We found that the sensitivity of IPCR was approximately over two order magnitude higher than that of conventional ELISA method. The result suggests that our IPCR system could be an applicable and reliable tool for early detection of NPC. ? 2008 Elsevier Ltd. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-50849128778&doi=10.1016%2fj.biomaterials.2008.07.015&partnerID=40&md5=b2118e590c2f82d4ed1d0c7e4bdc2a7b https://scholars.lib.ntu.edu.tw/handle/123456789/518292 |
ISSN: | 0142-9612 | DOI: | 10.1016/j.biomaterials.2008.07.015 | SDG/關鍵字: | Atomic force microscopy; Atomic spectroscopy; Chemical sensors; Chlorine compounds; Concentration (process); Contact angle; Cosmic rays; DNA; Food additives; Genes; Glass; Imaging techniques; Microscopic examination; Molecular orbitals; Molecular spectroscopy; Nucleic acids; Organic acids; Photoelectron spectroscopy; Photoresists; Scanning probe microscopy; Sensors; Surface chemistry; Surface properties; X ray photoelectron spectroscopy; Antibody conjugation; Biotinylated; Cancer; Capture system; Detection efficiencies; Detection systems; DNA concentration; Early detection; Enzyme substrate; Glass beads; Glass substrates; Glass surfaces; Highly sensitive; Immuno-PCR; Immunoassay; Model systems; Modified substrates; Nasopharyngeal carcinoma; Physical Properties; Pow erful tool; Silane; Surface modification; Surface modifications; Water contact angle; Substrates; antibody conjugate; DNA antibody; glass; article; atomic force microscopy; cancer diagnosis; cancer staging; early diagnosis; enzyme linked immunosorbent assay; enzyme substrate complex; feasibility study; human; nasopharynx carcinoma; polymerase chain reaction; priority journal; sensitivity and specificity; surface property; X ray photoelectron spectroscopy; Carcinoma; Early Diagnosis; Feasibility Studies; Glass; Humans; Immunoenzyme Techniques; Nasopharyngeal Neoplasms; Polymerase Chain Reaction; Sensitivity and Specificity; Substrate Specificity |
顯示於: | 醫學系 |
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