Stroke increases G protein-coupled estrogen receptor expression in the brain of male but not female mice
Journal
NeuroSignals
Journal Volume
21
Journal Issue
43894
Pages
229-239
Date Issued
2013
Author(s)
Broughton B.R.S.
Brait V.H.
Guida E.
Lee S.
Arumugam T.V.
Gardiner-Mann C.V.
Miller A.A.
Drummond G.R.
Sobey C.G.
Abstract
The novel estrogen receptor, G protein-coupled estrogen receptor (GPER, previously named GPR30), is widely distributed throughout the male and female brain and, thus, could potentially play a role in estrogen-mediated neuroprotective effects in diseases such as stroke. We hypothesized that GPER distribution and expression in the brain of male, intact female, and ovariectomized (OVX) mice is increased after 0.5 h middle cerebral artery occlusion. Using immunohistochemistry, we found that ischemia reperfusion increased GPER distribution in the peri-infarct brain regions of male mice, but surprisingly not in intact females or OVX mice. Similar differences were observed in the male and female human brain after stroke. In contrast, GPER distribution was decreased in the infarct core of all mice examined. Furthermore, GPER immunofluorescence was co-localized with the endothelial cell marker, von Willebrand factor, and the neuronal marker, NeuN. Consistent with the immunohistochemical findings, Western blot analysis showed GPER expression is only elevated in the ischemic hemisphere of male mice. Moreover, GPER mRNA expression in males was elevated at 4 h but had returned to baseline by 24 h. In conclusion, these findings indicate that GPER may be a potential therapeutic target after stroke, especially in males, in whom estrogen therapy is not feasible. ? 2012 S. Karger AG, Basel.
SDGs
Other Subjects
G protein coupled receptor 30; ganciclovir; misoprostol; von Willebrand factor; adult; animal experiment; animal model; aplastic anemia; Article; bone marrow biopsy; brain hemorrhage; brain ischemia; brain region; case report; cerebrovascular accident; child; controlled study; endothelium cell; female; follow up; hepatosplenomegaly; human; human tissue; hypothalamus; immunofluorescence; immunohistochemistry; immunoreactivity; male; middle aged; middle cerebral artery occlusion; mouse; nonhuman; ovariectomy; pancytopenia; preschool child; protein expression; protein localization; real time polymerase chain reaction; reperfusion injury; sex difference; somatosensory cortex; stroke patient; subarachnoid hemorrhage
Publisher
S. Karger AG
Type
journal article