https://scholars.lib.ntu.edu.tw/handle/123456789/519866
標題: | Eight-channel AC magnetosusceptometer of magnetic nanoparticles for high-throughput and ultra-high-sensitivity immunoassay | 作者: | Chieh J.-J. Wei W.-C. Liao S.-H. Chen H.-H. Lee Y.-F. Lin F.-C. Chiang M.-H. MING-JANG CHIU Horng H.-E. Yang S.-Y. |
關鍵字: | Immunoassay; Magnetic nanoparticle; Superconducting quantum interference device | 公開日期: | 2018 | 出版社: | MDPI AG | 卷: | 18 | 期: | 4 | 起(迄)頁: | 1043 | 來源出版物: | Sensors (Switzerland) | 摘要: | An alternating-current magnetosusceptometer of antibody-functionalized magnetic nanoparticles (MNPs) was developed for immunomagnetic reduction (IMR). A high-sensitivity, high-critical-temperature superconducting quantum interference device was used in the magnetosusceptometer. Minute levels of biomarkers of early-stage neurodegeneration diseases were detectable in serum, but measuring each biomarker required approximately 4 h. Hence, an eight-channel platform was developed in this study to fit minimal screening requirements for Alzheimer’s disease. Two consistent results were measured for three biomarkers, namely Aβ40, Aβ42, and tau protein, per human specimen. This paper presents the instrument configuration as well as critical characteristics, such as the low noise level variations among channels, a high signal-to-noise ratio, and the coefficient of variation for the biomarkers’ IMR values. The instrument’s ultrahigh sensitivity levels for the three biomarkers and the substantially shorter total measurement time in comparison with the previous single- and four-channels platforms were also demonstrated in this study. Thus, the eight-channel instrument may serve as a powerful tool for clinical high-throughput screening of Alzheimer’s disease. ? 2018 by the authors. Licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85044947118&doi=10.3390%2fs18041043&partnerID=40&md5=4125c52839e0a1d23875dc32e6463045 https://scholars.lib.ntu.edu.tw/handle/123456789/519866 |
ISSN: | 1424-8220 | DOI: | 10.3390/s18041043 | SDG/關鍵字: | Diagnosis; Immunology; Nanomagnetics; Nanoparticles; Neurodegenerative diseases; Quantum interference devices; Signal to noise ratio; SQUIDs; Superconducting devices; Throughput; Coefficient of variation; High critical temperature; High signal-to-noise ratio; High throughput screening; Immunoassay; Magnetic nano-particles; Magnetic nanoparti cles (MNPs); Ultra-high-sensitivity; Biomarkers; amyloid beta protein; biological marker; magnetite nanoparticle; tau protein; Alzheimer disease; human; immunoassay; magnetism; Alzheimer Disease; Amyloid beta-Peptides; Biomarkers; Humans; Immunoassay; Magnetics; Magnetite Nanoparticles; tau Proteins |
顯示於: | 醫學系 |
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