|Title:||Eight-channel AC magnetosusceptometer of magnetic nanoparticles for high-throughput and ultra-high-sensitivity immunoassay||Authors:||Chieh J.-J.
|Keywords:||Immunoassay; Magnetic nanoparticle; Superconducting quantum interference device||Issue Date:||2018||Publisher:||MDPI AG||Journal Volume:||18||Journal Issue:||4||Start page/Pages:||1043||Source:||Sensors (Switzerland)||Abstract:||
An alternating-current magnetosusceptometer of antibody-functionalized magnetic nanoparticles (MNPs) was developed for immunomagnetic reduction (IMR). A high-sensitivity, high-critical-temperature superconducting quantum interference device was used in the magnetosusceptometer. Minute levels of biomarkers of early-stage neurodegeneration diseases were detectable in serum, but measuring each biomarker required approximately 4 h. Hence, an eight-channel platform was developed in this study to fit minimal screening requirements for Alzheimer’s disease. Two consistent results were measured for three biomarkers, namely Aβ40, Aβ42, and tau protein, per human specimen. This paper presents the instrument configuration as well as critical characteristics, such as the low noise level variations among channels, a high signal-to-noise ratio, and the coefficient of variation for the biomarkers’ IMR values. The instrument’s ultrahigh sensitivity levels for the three biomarkers and the substantially shorter total measurement time in comparison with the previous single- and four-channels platforms were also demonstrated in this study. Thus, the eight-channel instrument may serve as a powerful tool for clinical high-throughput screening of Alzheimer’s disease. ? 2018 by the authors. Licensee MDPI, Basel, Switzerland.
|ISSN:||1424-8220||DOI:||10.3390/s18041043||SDG/Keyword:||Diagnosis; Immunology; Nanomagnetics; Nanoparticles; Neurodegenerative diseases; Quantum interference devices; Signal to noise ratio; SQUIDs; Superconducting devices; Throughput; Coefficient of variation; High critical temperature; High signal-to-noise ratio; High throughput screening; Immunoassay; Magnetic nano-particles; Magnetic nanoparti cles (MNPs); Ultra-high-sensitivity; Biomarkers; amyloid beta protein; biological marker; magnetite nanoparticle; tau protein; Alzheimer disease; human; immunoassay; magnetism; Alzheimer Disease; Amyloid beta-Peptides; Biomarkers; Humans; Immunoassay; Magnetics; Magnetite Nanoparticles; tau Proteins
|Appears in Collections:||醫學系|
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