R26R-GR: A Cre-Activable Dual Fluorescent Protein Reporter Mouse
Journal
PLoS ONE
Journal Volume
7
Journal Issue
9
Date Issued
2012
Author(s)
Tsai M.-S.
Ku A.T.
Huang K.-H.
Huang C.-Y.
Chou F.-J.
Fan H.-H.
Yen S.-T.
Wang W.-L.
Lin C.-C.
Hsu Y.-C.
Su I.-C.
Jang C.-W.
Behringer R.R.
Favaro R.
Nicolis S.K.
Yu I.-S.
Abstract
Green fluorescent protein (GFP) and its derivatives are the most widely used molecular reporters for live cell imagining. The development of organelle-specific fusion fluorescent proteins improves the labeling resolution to a higher level. Here we generate a R26 dual fluorescent protein reporter mouse, activated by Cre-mediated DNA recombination, labeling target cells with a chromatin-specific enhanced green fluorescence protein (EGFP) and a plasma membrane-anchored monomeric cherry fluorescent protein (mCherry). This dual labeling allows the visualization of mitotic events, cell shapes and intracellular vesicle behaviors. We expect this reporter mouse to have a wide application in developmental biology studies, transplantation experiments as well as cancer/stem cell lineage tracing.
Type
journal article