https://scholars.lib.ntu.edu.tw/handle/123456789/521278
標題: | Genome-wide DNA methylation profiles in hepatocellular carcinoma | 作者: | Shen J. Wang S. Zhang Y.-J. Kappil M. Wu H.-C. Kibriya M.G. Wang Q. Jasmine F. Ahsan H. PO-HUANG LEE MING-WHEI YU Chen C.-J. Santella R.M. |
公開日期: | 2012 | 卷: | 55 | 期: | 6 | 起(迄)頁: | 1799-1808 | 來源出版物: | Hepatology | 摘要: | Alterations in DNA methylation frequently occur in hepatocellular cancer (HCC). We have previously demonstrated that hypermethylation in candidate genes can be detected in plasma DNA before HCC diagnosis. To identify, with a genome-wide approach, additional genes hypermethylated in HCC that could be used for more accurate analysis of plasma DNA for early diagnosis, we analyzed tumor and adjacent nontumor tissues from 62 Taiwanese HCC cases using Illumina methylation arrays (Illumina, Inc., San Diego, CA) that screen 26,486 autosomal CpG sites. After Bonferroni adjustment, a total of 2,324 CpG sites significantly differed in methylation level, with 684 CpG sites significantly hypermethylated and 1,640 hypomethylated in tumor, compared to nontumor tissues. Array data were validated with pyrosequencing in a subset of five of these genes; correlation coefficients ranged from 0.92 to 0.97. Analysis of plasma DNA from 38 cases demonstrated that 37%-63% of cases had detectable hypermethylated DNA (?5% methylation) for these five genes individually. At least one of these genes was hypermethylated in 87% of the cases, suggesting that measurement of DNA methylation in plasma samples is feasible. Conclusion: The panel of methylated genes indentified in the current study will be further tested in a large cohort of prospectively collected samples to determine their utility as early biomarkers of HCC. ? 2012 American Association for the Study of Liver Diseases. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84861572881&doi=10.1002%2fhep.25569&partnerID=40&md5=8a9afff323849bf259988b1dbcfdba8c https://scholars.lib.ntu.edu.tw/handle/123456789/521278 |
ISSN: | 0270-9139 | DOI: | 10.1002/hep.25569 | SDG/關鍵字: | adult; article; autosome; cancer diagnosis; controlled study; correlation coefficient; CpG island; diagnostic accuracy; DNA determination; DNA methylation; early diagnosis; feasibility study; female; genetic association; human; human tissue; liver cell carcinoma; major clinical study; male; priority journal; pyrosequencing; Adult; Aged; Carcinoma, Hepatocellular; CpG Islands; DNA Methylation; Female; Genome-Wide Association Study; Humans; Liver Neoplasms; Male; Middle Aged |
顯示於: | 醫學系 |
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