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  1. NTU Scholars
  2. 醫學院
  3. 醫學系
Please use this identifier to cite or link to this item: https://scholars.lib.ntu.edu.tw/handle/123456789/521430
Title: The hDAF Exogene Protects Swine Endothelial and Peripheral Blood Mononuclear Cells From Xenoreactive Antibody Mediated Cytotoxicity in hDAF Transgenic Pigs
Authors: JANG-MING LEE 
Tu C.-F.
HAO-CHIH TAI 
NAI-KUAN CHOU 
Yang T.-S.
Weng C.-N.
Lee Y.-C.
Lee C.-J.
PO-HUANG LEE 
Issue Date: 2006
Journal Volume: 38
Journal Issue: 7
Start page/Pages: 2270-2272
Source: Transplantation Proceedings
Abstract: 
Xenoreactive antibody-induced complement activation and cytotoxicity poses a major obstracle to xenograft survival in humans. Previously, we have generated transgenic pigs carrying the hDAF exogene to help overcome this problem. In this study, we examined whether the hDAF exogene in various swine cells shows an equally protective effect for the complement-mediated cytotoxicity induced by human xenoreactive antibodies. Pig peripheral blood mononuclear cells (PBMCs) and aortic endothelial cells (PAECs) were used as targets. Fresh human serum was harvested from a single healthy human donor as the source of human xenoreactive antibodies and complement. The target cells cocultured with medium containing various concentrations of human serum for 24 hours were evaluated using the 3-[4,5-dimethylthiaolyl]-2,5-diphenyl-tetrazolium bromide assay for cellular viability. We observed that xenoreactive antibody plus human complement-mediated cellular cytoxicity dose-dependently correlated with the concentration of human serum in the culture medium. As compared with the PBMCs from the normal pigs, PBMCs from hDAF transgenic pigs showed significantly better survival after treatment with human serum (P < .05). Similarly, the survival of PAECs from the hDAF transgenic pig were also significantly higher than that from normal pigs (P < .05). Our data demonstrated a protective effect from human xenoreactive antibodies and complement-mediated cytoxicity of hDAF exogenes in pig PBMCs and PAECs. These observations support the clinical value of the hDAF transgenic pig as an organ donor in xenotransplantation. ? 2006 Elsevier Inc. All rights reserved.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-33845965965&doi=10.1016%2fj.transproceed.2006.06.079&partnerID=40&md5=07afa1f4250154b470b90e6853f00dc3
https://scholars.lib.ntu.edu.tw/handle/123456789/521430
ISSN: 0041-1345
DOI: 10.1016/j.transproceed.2006.06.079
SDG/Keyword: 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide; antibody detection; antibody production; article; cell survival; complement dependent cytotoxicity; diagnostic value; endothelium cell; evaluation research; exogene; hDAF gene; immunoreactivity; nonhuman; peripheral blood mononuclear cell; priority journal; transgenic animal; xenotransplantation; Animals; Animals, Genetically Modified; Antibodies, Heterophile; Antigens, CD; Antigens, CD55; Aorta; Cytotoxicity, Immunologic; Endothelium, Vascular; Humans; Leukocytes, Mononuclear; Swine
[SDGs]SDG3
Appears in Collections:醫學系

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臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

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