https://scholars.lib.ntu.edu.tw/handle/123456789/522757
標題: | The expression of the human chorionic gonadotropin beta subunit gene depends on negative control | 作者: | YEN-HUI CHEN Chen T.-M. CHANG-YAO HSIEH |
公開日期: | 1996 | 出版社: | John Wiley and Sons Ltd | 卷: | 14 | 期: | 4 | 起(迄)頁: | 297-301 | 來源出版物: | Cell Biochemistry and Function | 摘要: | Transcription of the human chorionic gonadotropin (hCG) genes begins in the very early embryo stage and decreases or even disappears in nonplacental tissues. We have studied the regulation of hCG-beta genes by cell fusion and by the reverse transcription-polymerase chain reaction (RT-PCR). The choriocarcinoma cell lines, JAR and JEG-3, express high levels of the hCG-beta subunit while HeLa cells express extremely low levels of it. Most HeLa x JAR and HeLa x JEG-3 fusion clones expressed only a trace of the hCG-beta subunit mRNA, while JAR x JEG-3 fusion clones still expressed high levels of the hCG-beta subunit, Most transcripts of the hCG-beta subunit genes in JAR and JEG-3 came from the hCG-beta 5 subunit. Even the trace amount of hCG-beta transcripts from fusion clones came mainly from the beta-5 gene. The results suggest that the expression of the hCG-beta subunit genes depends on negative control. Probably when embryonic cells differentiate to form nonplacental tissues, specific inhibitors may appear and inhibit the expression of the hCG-beta subunit genes. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029844709&doi=10.1002%2fcbf.686&partnerID=40&md5=d8c14dbc02a6030ee76e3b3198a4ec4c https://scholars.lib.ntu.edu.tw/handle/123456789/522757 |
ISSN: | 0263-6484 | DOI: | 10.1002/cbf.686 | SDG/關鍵字: | chorionic gonadotropin beta subunit; messenger RNA; article; cancer cell culture; cell fusion; choriocarcinoma; controlled study; gene repression; HeLa cell; human; human cell; priority journal; reverse transcription polymerase chain reaction |
顯示於: | 醫學系 |
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