https://scholars.lib.ntu.edu.tw/handle/123456789/523133
標題: | Non-invasive Drosophila ECG recording by using eutectic gallium-indium alloy electrode: A feasible tool for future research on the molecular mechanisms involved in cardiac arrhythmia | 作者: | Kuo P.-H. Tzeng T.-H. Huang Y.-C. Chen Y.-H. Chang Y.-C. YI-LWUN HO Wu J.-T. Lee H.-H. Lai P.-J. Liu K.-Y. Cheng Y.-C. Lu S.-S. |
公開日期: | 2014 | 出版社: | Public Library of Science | 卷: | 9 | 期: | 9 | 來源出版物: | PLoS ONE | 摘要: | Background: Drosophila heart tube is a feasible model for cardiac physiological research. However, obtaining Drosophila electrocardiograms (ECGs) is difficult, due to the weak signals and limited contact area to apply electrodes. This paper presents a non-invasive Gallium-Indium (GaIn) based recording system for Drosophila ECG measurement, providing the heart rate and heartbeat features to be observed. This novel, high-signal-quality system prolongs the recording time of insect ECGs, and provides a feasible platform for research on the molecular mechanisms involved in cardiovascular diseases. Methods: In this study, two types of electrode, tungsten needle probes and GaIn electrodes, were used respectively to noiselessly conduct invasive and noninvasive ECG recordings of Drosophila. To further analyze electrode properties, circuit models were established and simulated. By using electromagnetic shielded heart signal acquiring system, consisted of analog amplification and digital filtering, the ECG signals of three phenotypes that have different heart functions were recorded without dissection. Results and Discussion: The ECG waveforms of different phenotypes of Drosophila recorded invasively and repeatedly with n value (n>5) performed obvious difference in heart rate. In long period ECG recordings, non-invasive method implemented by GaIn electrodes acts relatively stable in both amplitude and period. To analyze GaIn electrode, the correctness of GaIn electrode model established by this paper was validated, presenting accuracy, stability, and reliability. Conclusions: Noninvasive ECG recording by GaIn electrodes was presented for recording Drosophila pupae ECG signals within a limited contact area and signal strength. Thus, the observation of ECG changes in normal and SERCA-depleted Drosophila over an extended period is feasible. This method prolongs insect survival time while conserving major ECG features, and provides a platform for electrophysiological signal research on the molecular mechanism involved in cardiac arrhythmia, as well as research related to drug screening and development. ? 2014 Kuo et al. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84907279782&doi=10.1371%2fjournal.pone.0104543&partnerID=40&md5=c71be9a9b57744cad7922828c9f43c33 https://scholars.lib.ntu.edu.tw/handle/123456789/523133 |
ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0104543 | SDG/關鍵字: | gallium; indium; tungsten; Article; controlled study; digital filtering; Drosophila; electrocardiography monitoring; electrode; electromagnetic field; feasibility study; heart arrhythmia; heart beat; heart rate; molecular mechanics; nonhuman; phenotype; pupa (life cycle stage); signal detection; simulation; waveform; animal; Arrhythmias, Cardiac; Drosophila; electrocardiography; pathophysiology; research; Animals; Arrhythmias, Cardiac; Drosophila; Electrocardiography; Electrodes; Research |
顯示於: | 醫學系 |
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