https://scholars.lib.ntu.edu.tw/handle/123456789/523670
標題: | Dynamic contrast-enhanced folate-receptor-targeted MR imaging using a gd-loaded peg-dendrimer-folate conjugate in a mouse xenograft tumor model | 作者: | Chen W.-T. Thirumalai D. TIFFANY TING-FANG SHIH Chen R.-C. Tu S.-Y. Lin C.-I. PAN-CHYR YANG |
公開日期: | 2010 | 卷: | 12 | 期: | 2 | 起(迄)頁: | 145-154 | 來源出版物: | Molecular Imaging and Biology | 摘要: | Purpose: The purpose of this study is to validate a folate-receptor (FR)-targeted dendrimer, PEG-G3-(Gd-DTPA)11-(folate)5, for its ability to detect FR-positive tumors, by using dynamic contrast-enhanced MRI. Procedures: KB cells, FR siRNA knockdown KB cells, and FR negative HT-1080 cells, were incubated with fluorescein-labeled dendrimer and their cellular uptake was observed. Dynamic contrast-enhanced MRI was performed on mice-bearing KB and HT-1080 tumors and the enhancement patterns and parameters were analyzed. Results: Green fluorescence was found in the KB cells in the cellular uptake experiment, but was not seen in other settings. In the dynamic contrast-enhanced MRI, the 30-min washout percentage was -4±18% in the KB tumors and 39±23% in the HT-1080 tumors. A 17% cut-off point gave a sensitivity of 94.4% and a specificity of 93.8%. Conclusions: We have demonstrated the targeting ability of PEG-G3-(Gd-DTPA)11-(folate)5 in vitro and in vivo. A 17% cut-off point for a 30-min washout percentage can be a useful parameter for the diagnosis of FR-positive tumors. ? Academy of Molecular Imaging, 2009. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-77953542134&doi=10.1007%2fs11307-009-0248-6&partnerID=40&md5=a61202dc184ae3f2567550677e7c633c https://scholars.lib.ntu.edu.tw/handle/123456789/523670 |
ISSN: | 1536-1632 | DOI: | 10.1007/s11307-009-0248-6 | SDG/關鍵字: | dendrimer; dicyclohexylcarbodiimide; dimethyl sulfoxide; folate receptor; folic acid; gadolinium pentetate derivative; macrogol; small interfering RNA; animal experiment; animal model; article; cell strain KB; contrast enhancement; controlled study; diagnostic value; drug dosage form comparison; drug structure; fluorescence analysis; human; human cell; immunohistochemistry; in vitro study; in vivo study; male; malignant neoplastic disease; mouse; nonhuman; nuclear magnetic resonance imaging; nuclear magnetic resonance spectroscopy; priority journal; reverse transcription polymerase chain reaction; sensitivity and specificity; tumor xenograft; Animals; Carrier Proteins; Cell Line, Tumor; Contrast Media; Dendrimers; Folic Acid; Gadolinium DTPA; Humans; Immunohistochemistry; Magnetic Resonance Imaging; Magnetic Resonance Spectroscopy; Mice; Polyethylene Glycols; Receptors, Cell Surface; Reverse Transcriptase Polymerase Chain Reaction; RNA, Small Interfering; Time Factors; Xenograft Model Antitumor Assays; Mus |
顯示於: | 醫學系 |
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