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  1. NTU Scholars
  2. 醫學院
  3. 醫學系
Please use this identifier to cite or link to this item: https://scholars.lib.ntu.edu.tw/handle/123456789/524008
Title: Detection and quantitation of circulating cancer cells in the peripheral blood of lung cancer patients
Authors: Peck K.
Sher Y.-P.
JIN-YUAN SHIH 
Roffler S.R.
Wu C.-W.
PAN-CHYR YANG 
Issue Date: 1998
Journal Volume: 58
Journal Issue: 13
Start page/Pages: 2761-2765
Source: Cancer Research
Abstract: 
Detection and quantitation of circulating cancer cells in peripheral blood may improve cancer staging and monitoring. This study explored the feasibility of using circulating cancer cell detection in peripheral blood for the rapid assessment of chemotherapeutic response. Cytokeratin 19 mRNA was amplified by nested reverse transcriptase-PCR in the peripheral blood of 29 healthy volunteers, 33 pneumonia patients, and 86 lung cancer patients. Circulating cancer cells in the peripheral blood were semiquantitatively determined by taking the ratio of cytokeratin 19 band intensity from the second round of nested PCR to the glyceraldehyde-3-phosphate dehydrogenase band intensity from the first round of PCR amplification. The detection limit of the method was 1 cancer cell in 107 peripheral blood mononuclear cells. The positive detection rate was 40% for lung adenocarcinoma patients of all stages, 41% for squamous carcinoma patients of all stages, and 27% for small cell lung cancer patientS. Only one control sample from a pneumonia patient showed a positive result (1.6%). The quantitative method reliably and sensitively estimated cancer cell numbers in the peripheral blood of lung cancer patients. Serial measurement of the relative number of circulating cancer cells correlated with the tumor burden and treatment response of patients. This method may help rapidly assess the efficacy of anticancer treatment, redefine cancer staging, and facilitate the design of better therapeutic strategies for the treatment of cancer patients.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032125583&partnerID=40&md5=84eaeaf158aeb2ba03d4419f7b8ce93b
https://scholars.lib.ntu.edu.tw/handle/123456789/524008
ISSN: 0008-5472
SDG/Keyword: cytokeratin 19; glyceraldehyde 3 phosphate dehydrogenase; accuracy; adult; aged; article; blood sampling; cancer cell; cancer diagnosis; cancer staging; controlled study; disease marker; drug response; female; human; human cell; human tissue; lung cancer; major clinical study; male; priority journal; quantitative assay; reverse transcription polymerase chain reaction; treatment planning; Adult; Aged; Aged, 80 and over; Feasibility Studies; Female; Humans; Keratins; Lung Neoplasms; Male; Middle Aged; Neoplasm Circulating Cells; Polymerase Chain Reaction; Sensitivity and Specificity; Tumor Markers, Biological
[SDGs]SDG3
Appears in Collections:醫學系

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臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

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開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

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